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Forum: Illumina/Solexa 09-10-2015, 10:03 PM
Replies: 12
Views: 7,535
Posted By Hyeshik Chang
The purpose of my library was a lot simpler than...

The purpose of my library was a lot simpler than the general use cases of specialized base calling. Mine was just for rough length measurement of homopolymers (poly(A) tails in mRNA). It was not very...
Forum: Illumina/Solexa 09-08-2015, 04:59 PM
Replies: 12
Views: 7,535
Posted By Hyeshik Chang
Hi ScottC, Thank you for the comment. I...

Hi ScottC,

Thank you for the comment. I could get CIF files from NextSeq by editing the line as you suggested. :) It looks that they did not change the file format of CIF files except the number...
Forum: Illumina/Solexa 07-21-2015, 09:46 PM
Replies: 12
Views: 7,535
Posted By Hyeshik Chang
Obtaining .CIF or raw images from HiSeq/NextSeq?

Hi,

Illumina recently changed the data flow inside their HiSeq and NextSeq machines to reduce I/O burden during sequencing dramatically. It now skips saving .TIFF image files and .CIF intensity...
Forum: Illumina/Solexa 07-21-2015, 09:29 PM
Replies: 10
Views: 7,242
Posted By Hyeshik Chang
Hi dorix, The file has been renamed to...

Hi dorix,

The file has been renamed to MiSeqSoftware.Options.cfg sometime ago.
Forum: Illumina/Solexa 07-02-2013, 05:34 PM
Replies: 2
Views: 2,275
Posted By Hyeshik Chang
Different number of cycles for HiSeq PE sequencing

Hello,

I am planning to run a HiSeq 2500 rapid run with a different number of cycles for reads 1 and 2 (e.g. 250 x 50). I know that this layout produces a lot worse quality than 150 x 150,...
Forum: Bioinformatics 07-10-2010, 08:27 PM
Replies: 2
Views: 1,629
Posted By Hyeshik Chang
How about trying de novo assembly of the sequence...

How about trying de novo assembly of the sequence reads?

See: http://bioinformatics.oxfordjournals.org/cgi/content/full/25/21/2872
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