Forum: Sample Prep / Library Generation
04-04-2018, 02:40 AM
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Replies: 5
Views: 3,475
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Forum: Sample Prep / Library Generation
12-17-2014, 12:25 AM
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Replies: 3
Views: 1,631
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Forum: Sample Prep / Library Generation
12-09-2014, 01:16 PM
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Replies: 3
Views: 1,631
PCR duplicates
Hello,
what is PCR duplicate in NGS sequencing? Is there a detailed explanation of this problem? What is the impact of PCR duplictes to data analysis.
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Forum: Sanger/Dye Terminator
11-19-2014, 01:45 AM
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Replies: 5
Views: 3,196
Do You always set sequencing reactions on...
Do You always set sequencing reactions on chemistry version 1.1?
Did You check the protocol on instrument that is set up for version 1.1 not for 3.1?I think there are different mobility files etc....
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Forum: Sample Prep / Library Generation
11-17-2014, 12:20 AM
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Replies: 68
Views: 25,975
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Forum: Sample Prep / Library Generation
11-14-2014, 12:34 AM
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Replies: 68
Views: 25,975
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Forum: Sample Prep / Library Generation
11-12-2014, 03:32 AM
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Replies: 68
Views: 25,975
Thanks for information. Is there a special...
Thanks for information. Is there a special protocol for preparing libraries from cffDNA and is this protocol include shearing DNA after extraction or cffDNA is ready to use as it is (200 bp fragments...
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Forum: Sample Prep / Library Generation
11-11-2014, 06:48 AM
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Replies: 68
Views: 25,975
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Forum: Sanger/Dye Terminator
11-11-2014, 06:27 AM
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Replies: 5
Views: 3,196
Hello,
in my opinion the average signal...
Hello,
in my opinion the average signal strenght for all bases is very low ( below 100 ) and the software pull up the background to maximaze the real signal thats why You get double signal but...
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Forum: Sanger/Dye Terminator
11-06-2014, 11:58 PM
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Replies: 1
Views: 2,492
low signal for one base
Sometimes i have problem with signal intensity for one base.The average signal for all bases is good. It is similar problem to G after A but it not always G that have very low signal.
Any...
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Forum: Sample Prep / Library Generation
11-06-2014, 02:59 PM
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Replies: 4
Views: 2,053
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Forum: Sample Prep / Library Generation
11-06-2014, 03:04 AM
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Replies: 4
Views: 2,053
NebNext Adaptors for illumina
Hi all,
anyone know wchich adapters are better for whole genome sequencing, I'm thinking about NebNext loop adapters. Are they better than standard Y shape adapters.?
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Forum: Vendor Forum
11-05-2014, 12:32 AM
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Replies: 3
Views: 7,270
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