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Forum: Sample Prep / Library Generation 07-28-2016, 11:03 PM
Replies: 7
Views: 6,997
Posted By Simone78
yes, unfortunately itīs like that. Tagmentation...

yes, unfortunately itīs like that. Tagmentation <1 ng DNA input doesnīt work very well with the Nextera kit.
Forum: Sample Prep / Library Generation 11-18-2015, 10:22 PM
Replies: 7
Views: 6,997
Posted By Simone78
Hi, I am not 100% sure but I think that the...

Hi,
I am not 100% sure but I think that the Illumina adaptors have a biotin at the 5ī-end. We donīt buy our own adaptors anymore but just order those from Illumina. We never tagment more than...
Forum: Sample Prep / Library Generation 02-14-2014, 12:40 AM
Replies: 7
Views: 6,997
Posted By Simone78
adaptors and primers Nextera kit: modified or not?

Hi,
I am using Nextera XT kit from Illumina but tried to order the adaptors from another vendor, since the sequence is disclosed. Unfortunately, I get sub-optimal results due to accumulation of...
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