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Forum: Sample Prep / Library Generation 10-12-2016, 06:48 AM
Replies: 289
Views: 155,262
Posted By JJMS
TSO in qPCR

Hi SunPenguin, yes we did. We are sorting single B cells and before we send for sequencing we apply a qPCR panel to test for T/B/NK presence. If the transcripts are there you'll find the expected...
Forum: Sample Prep / Library Generation 10-12-2015, 12:24 AM
Replies: 289
Views: 155,262
Posted By JJMS
Hi Simone, Yes it sure looks like that. Our...

Hi Simone,
Yes it sure looks like that. Our profiles with high input and SMARTscribe indeed match ssII nearly to a 100%. But with single cells SMARTscribe deviates a little. I have to leave out Mg...
Forum: Sample Prep / Library Generation 10-02-2015, 01:53 AM
Replies: 289
Views: 155,262
Posted By JJMS
Clontech SMARTscribe

Hello everybody and especially Wishingfly,

I followed your advice on complaining to Life Technologies about the SSII contamination. Their European facility also distributes contaminated...
Forum: Sample Prep / Library Generation 08-03-2014, 11:43 AM
Replies: 289
Views: 155,262
Posted By JJMS
AMpure before cDNA amplification

Did somebody ever use the Ampure purification in between the 1st strand synthesis and cDNA amplification in the SMARTseq2 protocol? (like Clontech has standard in it's ultra-low protocol)
Forum: Bioinformatics 01-21-2013, 03:53 AM
Replies: 24
Views: 8,118
Posted By JJMS
Had the same problem... Solved it by reinstalling...

Had the same problem... Solved it by reinstalling the software with option "Local" instead of "system" somewhere in the beginning of the instalation
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