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Forum: 454 Pyrosequencing 06-19-2008, 11:09 AM
Replies: 61
Views: 93,934
Posted By seqer
$5000 total or each? It is hard to imagine they...

$5000 total or each? It is hard to imagine they are doing $1000 each - the array would cost at least $600.
Forum: 454 Pyrosequencing 02-28-2008, 08:58 PM
Replies: 61
Views: 93,934
Posted By seqer
Thanks sci_guy and ECO! This thing is damn...

Thanks sci_guy and ECO! This thing is damn expensive... For some reason, people believe physical shearing / sonication is less biased than any enzymatic reaction. It might be worth trying DNase. ...
Forum: 454 Pyrosequencing 02-28-2008, 08:09 PM
Replies: 61
Views: 93,934
Posted By seqer
It seems to me that shearing the genomic DNA is...

It seems to me that shearing the genomic DNA is quite critical. If one can reliably shear DNA to ~100bp or 200bp, the genomic DNA will move faster (thus increase hybridization efficiency), and the...
Forum: 454 Pyrosequencing 02-07-2008, 08:17 PM
Replies: 61
Views: 93,934
Posted By seqer
Very good points colicoli! I still dont quite...

Very good points colicoli! I still dont quite understand hyb at 42C. It seems that the specificity looks okay (at least in Albert et al). Intuitively, 42C hyb would generate a lot of nonspecific...
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