Forum: Illumina/Solexa
02-19-2019, 07:46 AM
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Replies: 0
Views: 1,590
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Forum: Sample Prep / Library Generation
01-04-2019, 07:01 AM
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Replies: 5
Views: 2,873
Hello,
I have found half a solution - the...
Hello,
I have found half a solution - the library preparation kit is not contaminated nor unstable. You were correct that degradation was not the issue. Unexpected as the declining qPCR readings...
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Forum: Sample Prep / Library Generation
11-16-2018, 06:42 AM
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Replies: 5
Views: 2,873
Additional images
I will try to attach images of the qPCR concentrations, as well as before and after images visualized by the Bioanalyzer.
image 1 - qPCR values
image 2 - post qPCR HighSense visualizations (lane...
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Forum: Sample Prep / Library Generation
11-16-2018, 06:23 AM
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Replies: 5
Views: 2,873
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Forum: Sample Prep / Library Generation
11-14-2018, 07:03 AM
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Replies: 5
Views: 2,873
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Forum: Sample Prep / Library Generation
11-13-2018, 08:57 AM
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Replies: 0
Views: 1,005
Library degradation of size select?
Hello,
I am having an issue with library pools rapidly degrading after pooling and size selection.
I am prepping low biomass samples with Nugen’s Ovation Ultralow Library System V2 kit. Samples...
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