SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 6 of 6
Search took 0.05 seconds.
Search: Posts Made By: robicha7
Forum: Illumina/Solexa 02-19-2019, 07:46 AM
Replies: 0
Views: 1,590
Posted By robicha7
Question NextSeq - proportional sequencing of different fragment sizes on the same run

Hello,

I'm still learning the boundaries of the NextSeq and was wondering if anyone had experience with the following or advice where I could find more information.

I am trying to optimize...
Forum: Sample Prep / Library Generation 01-04-2019, 07:01 AM
Replies: 5
Views: 2,873
Posted By robicha7
Hello, I have found half a solution - the...

Hello,

I have found half a solution - the library preparation kit is not contaminated nor unstable. You were correct that degradation was not the issue. Unexpected as the declining qPCR readings...
Forum: Sample Prep / Library Generation 11-16-2018, 06:42 AM
Replies: 5
Views: 2,873
Posted By robicha7
Additional images

I will try to attach images of the qPCR concentrations, as well as before and after images visualized by the Bioanalyzer.

image 1 - qPCR values
image 2 - post qPCR HighSense visualizations (lane...
Forum: Sample Prep / Library Generation 11-16-2018, 06:23 AM
Replies: 5
Views: 2,873
Posted By robicha7
Yes, the library pool degrades after size...

Yes, the library pool degrades after size selection and clean up while in the 4C fridge.
Forum: Sample Prep / Library Generation 11-14-2018, 07:03 AM
Replies: 5
Views: 2,873
Posted By robicha7
Question Library degrading after prep with Ovation Ultralow V2

Hello,

I am having an issue with library pools rapidly degrading after pooling and size selection.

I am prepping low biomass samples with Nugen’s Ovation Ultralow Library System V2 kit. Samples...
Forum: Sample Prep / Library Generation 11-13-2018, 08:57 AM
Replies: 0
Views: 1,005
Posted By robicha7
Library degradation of size select?

Hello,

I am having an issue with library pools rapidly degrading after pooling and size selection.

I am prepping low biomass samples with Nugenís Ovation Ultralow Library System V2 kit. Samples...
Showing results 1 to 6 of 6

 


All times are GMT -8. The time now is 05:39 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO