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Forum: Sample Prep / Library Generation 08-12-2019, 07:10 AM
Replies: 20
Views: 5,909
Posted By lapensee
Halt protease inhib without EDTA (100x) from...

Halt protease inhib without EDTA (100x) from thermofisher
Forum: Sample Prep / Library Generation 04-03-2019, 04:45 AM
Replies: 20
Views: 5,909
Posted By lapensee
I used "Nextera DNA Library Prep Kit" ...

I used "Nextera DNA Library Prep Kit"
FC-121-1030
Forum: Sample Prep / Library Generation 03-20-2019, 10:16 AM
Replies: 20
Views: 5,909
Posted By lapensee
FWIW I got this working. I adopted the OMNI-ATAC...

FWIW I got this working. I adopted the OMNI-ATAC protocol and get great library amplification from 5k cells using only 2% the amount of Tn5 required for 50k cells. Adding a protease inhibitor during...
Forum: Sample Prep / Library Generation 12-13-2018, 11:15 AM
Replies: 20
Views: 5,909
Posted By lapensee
Undertagmentation

Suspecting undertagmentation. Anyone else run into this problem, and if so, what was your remedy?
Forum: Sample Prep / Library Generation 12-03-2018, 08:41 AM
Replies: 20
Views: 5,909
Posted By lapensee
If not suggestions, would someone be willing to...

If not suggestions, would someone be willing to post a pic of a GREAT bioanalyzer profile (for ATAC-Seq)?
Forum: Sample Prep / Library Generation 11-29-2018, 05:27 AM
Replies: 20
Views: 5,909
Posted By lapensee
Inefficient ATAC-seq library prep amplification

Hi all,

I’m having trouble generating an “optimal” library for sequencing.
I am using a human cell line derived from an adrenocortical tumor (from ATCC).

I have tried 12.5, 25, 50, and 100k...
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