Forum: Sample Prep / Library Generation
03-28-2013, 12:08 PM
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Replies: 49
Views: 73,821
As mentioned earlier with Ampure XP, we were...
As mentioned earlier with Ampure XP, we were seeing inconsistent results. Our recent testing with SPRIselect has been going well and the data so far looks amazing! What seem to work best for us is...
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Forum: Sample Prep / Library Generation
03-26-2013, 12:59 PM
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Replies: 49
Views: 73,821
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Forum: Illumina/Solexa
12-17-2012, 10:23 AM
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Replies: 32
Views: 26,464
We just got our machine upgraded to a 2500....
We just got our machine upgraded to a 2500. Validation runs went great and we will be running two FCs today on Rapid Mode! Nice not having to wait to fill FCs when odd read lengths are requested....
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Forum: Events / Conferences
12-17-2012, 09:52 AM
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Replies: 1
Views: 1,384
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Forum: Illumina/Solexa
07-06-2012, 02:26 PM
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Replies: 9
Views: 4,784
Hi Phillip,
We have used BiooScientific...
Hi Phillip,
We have used BiooScientific Adapters (96 are available). Since you are at a core you understand the difficulty in batching samples/orders. Also, if the library failure rate is low and...
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Forum: Illumina/Solexa
07-06-2012, 12:44 PM
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Replies: 9
Views: 4,784
MiSeq for library QC
Hello,
Are you at a core facility that has a MiSeq? If so, are you using the MiSeq to quantify/QC libraries before processing on a HiSeq? If you do this, can you share how you have the workflow...
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Forum: General
02-21-2012, 10:44 AM
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Replies: 1
Views: 13,733
Conferences
http://conf.abrf.org/index.cfm/page/satellite_workshops.htm
Check out the ABRF conference in March. There is a pre-meeting workshop on RNA-Seq and Methyl-Seq
There is also the xgenconference:...
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Forum: RNA Sequencing
02-03-2012, 02:19 PM
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Replies: 0
Views: 1,794
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Forum: Epigenetics
02-03-2012, 02:18 PM
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Replies: 0
Views: 2,198
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Forum: Sample Prep / Library Generation
12-14-2011, 07:36 AM
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Replies: 1
Views: 1,778
DNA/RNA analyzing
We are considering buying a high throughput DNA/RNA analyzer. So far we have looked at Caliper LabChip GX and Advanced Analytical Fragment Analyzer. Soon we will be looking at the Agilent Tape...
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Forum: Sample Prep / Library Generation
09-28-2011, 09:57 AM
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Replies: 13
Views: 5,900
Just a quick update...
We tried the Millipore...
Just a quick update...
We tried the Millipore Magna ChIP-Seq kit with the Bioo Scientific adapters, and obtained beautiful libraries. We have only constructed 10 libraries so far, and hope to...
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Forum: Sample Prep / Library Generation
06-13-2011, 10:20 AM
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Replies: 13
Views: 5,900
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Forum: Sample Prep / Library Generation
05-24-2011, 11:37 AM
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Replies: 13
Views: 5,900
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Forum: Sample Prep / Library Generation
05-12-2011, 01:09 PM
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Replies: 13
Views: 5,900
Illumina ChIP-Seq
What kits are people currently using to make Illumina multiplexed ChIP-Seq libraries? We are not able to get an answer from Illumina regarding when the TruSeq ChIP-Seq kits will be available or what...
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Forum: Epigenetics
10-08-2010, 12:08 PM
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Replies: 5
Views: 4,180
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Forum: Illumina/Solexa
10-05-2010, 08:19 AM
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Replies: 4
Views: 4,667
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Forum: Illumina/Solexa
10-04-2010, 03:30 PM
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Replies: 4
Views: 4,667
LIMS for Next gen sequencing
Currently we have a custom LIMS that require some updates. We are considering if we should make these changes or purchase a commercial LIMS. As for commercial LIMS we have been looking at the ones...
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Forum: Sample Prep / Library Generation
10-04-2010, 03:28 PM
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Replies: 1
Views: 2,808
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Forum: Illumina/Solexa
09-27-2010, 11:42 AM
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Replies: 18
Views: 7,246
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Forum: Illumina/Solexa
09-24-2010, 10:27 AM
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Replies: 18
Views: 7,246
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Forum: Sample Prep / Library Generation
08-03-2010, 08:59 AM
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Replies: 0
Views: 2,272
Illumina Library Prep Issue
Recently, we made 4 Illumina paired-end libraries using the standard protocol with a brand new Illumina paired-end sample prep kit. The libraries looked great on a bioanalyzer but when we sequenced,...
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Forum: Sample Prep / Library Generation
08-03-2010, 08:35 AM
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Replies: 23
Views: 18,605
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Forum: Sample Prep / Library Generation
08-03-2010, 08:29 AM
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Replies: 6
Views: 5,146
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