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Forum: Bioinformatics 09-29-2015, 10:39 AM
Replies: 0
Views: 1,691
Posted By melis
read number in fastq does not match bwa-mem produced sam file

Hi all,

I used bwa mem to align my quality trimmed reads to a reference. I have 5M reads to align but in the sam file there are only 3M of them included (I grep the read names' initial part and...
Forum: Sample Prep / Library Generation 04-29-2014, 05:53 PM
Replies: 5
Views: 1,515
Posted By melis
I would suggest not to ppol your samples but do a...

I would suggest not to ppol your samples but do a multiplexed library. Good luck!
Forum: Bioinformatics 04-29-2014, 11:01 AM
Replies: 1
Views: 3,261
Posted By melis
Angry Orthomcl Pairs error

Hello everyone,

I am trying to get orthomcl running for my non-model organism and I am using cDNA sequences for 6 species in total. I created a blastdb index with the goodproteins.fasta created...
Forum: RNA Sequencing 01-20-2012, 04:08 PM
Replies: 6
Views: 6,621
Posted By melis
Hi all, I am also having the same problem. My...

Hi all,
I am also having the same problem. My ID format is the same but still I get the same error when I run this.
> stats=goseq(pwf,gene2cat=go.terms,method='Wallenius')
Using manually entered...
Forum: Bioinformatics 01-19-2012, 06:42 AM
Replies: 1
Views: 2,744
Posted By melis
Unhappy GO analysis for Arabidopsis with Goseq

Hi all,
I am trying to do a GO analysis for Arabidopsis RNA-seq data with Goseq. The software needs gene length information for all the genes but I cannot find it anywhere. I used the...
Forum: Introductions 01-04-2012, 01:40 PM
Replies: 373
Views: 138,322
Posted By melis
Hi folks, I am a PhD student from Amsterdam,...

Hi folks,
I am a PhD student from Amsterdam, almost finishing (I hope).
Happy new year to you all!
Melis
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