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Forum: General 07-15-2019, 03:49 PM
Replies: 3
Views: 4,486
Posted By seqsuave
Has anyone tried half-reactions for the QuantSeq...

Has anyone tried half-reactions for the QuantSeq chemistry?
Forum: Sample Prep / Library Generation 05-23-2019, 02:47 PM
Replies: 12
Views: 1,474
Posted By seqsuave
How fresh was the 80% Ethanol, if ethanol...

How fresh was the 80% Ethanol, if ethanol evaporated off you could have had elution during the washes. It is recommended that 80% Ethanol is not more than a week old.
Forum: Sample Prep / Library Generation 05-22-2019, 02:48 PM
Replies: 0
Views: 598
Posted By seqsuave
10x Genomics Single-cell Half Reactions

Has anyone attempted or successfully done half reactions for 10x Genomics platforms?

I know that the Gene Expression kits can capture 500-10,000 cells, but when you just need 500-5,000 cells/...
Forum: Sample Prep / Library Generation 03-14-2019, 01:59 PM
Replies: 10
Views: 1,226
Posted By seqsuave
Why are you working at 37C? Most TS I have seen...

Why are you working at 37C? Most TS I have seen is around 50C, this might help to prevent any mismatching of your TSO or RT primers.
Forum: Sample Prep / Library Generation 03-14-2019, 01:50 PM
Replies: 1
Views: 739
Posted By seqsuave
Most Versatile DNA Prep Kit

Hello Everyone!

I am interested in peoples' opinion on what they would consider to be the most versatile DNA prep kit/enzymes. Most DNA library prep methods are pretty consistent in terms of...
Forum: Sample Prep / Library Generation 03-14-2019, 01:35 PM
Replies: 1
Views: 1,312
Posted By seqsuave
AmpliSeq vs TruSeq

Now that Illumina sells AmpliSeq, I am trying understanding the difference between AmpliSeq for Illumina and TruSeq Targeted panels. From what I can tell AmpliSeq can probe for more targets, and deal...
Forum: Ion Torrent 05-09-2018, 02:46 PM
Replies: 8
Views: 6,280
Posted By seqsuave
I am looking for cheaper kits for the PGM for...

I am looking for cheaper kits for the PGM for around the same purpose, and while I have not done any testing yet, I found some kits that have barcodes included so you done have to worry about Ion...
Forum: Ion Torrent 05-01-2018, 02:33 PM
Replies: 9
Views: 6,622
Posted By seqsuave
Thats interesting and really cool, thanks for...

Thats interesting and really cool, thanks for that.
I didn't know the upgraded the S5, I wonder how the list prices compare to the S5 or S5 XL.

I have used S5 before, its a nice system I like it...
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