Forum: Sanger/Dye Terminator
12-29-2015, 08:54 PM
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Replies: 3
Views: 6,091
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Forum: Sanger/Dye Terminator
12-28-2015, 10:23 PM
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Replies: 3
Views: 6,091
Rerun of cycle sequencing
Hi All
Recently I have run a cycle sequencing reaction, however the run was stopped due to a fatal error in the thermocycler. it stopped at cycle 8 of 25 cycles for approximately an hour till I...
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Forum: Sanger/Dye Terminator
12-16-2015, 10:37 PM
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Replies: 1
Views: 5,955
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Forum: Sanger/Dye Terminator
12-14-2015, 10:13 PM
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Replies: 1
Views: 5,955
3130 genetic analyzer raw data range
hi
Well for those who have used 3130 genetic analyzer, when you look in the raw data of a run , how do you interrupt the results?
in my case , most of the time I got a raw data signal...
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Forum: Sanger/Dye Terminator
12-14-2015, 09:53 PM
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Replies: 4
Views: 6,822
Viroseq sequencing
hi Ivoalexiev
Well so far we find out that we have problem in measuring the right concentration of the PCR purified products, which is very critical in cycle sequencing reactions . Unfortunately,...
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Forum: Sanger/Dye Terminator
11-11-2015, 02:58 AM
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Replies: 4
Views: 6,822
ViroSeq sequencing
hi Ivoalexiev
Good to know that you are using ViroSeq for resistance studies. Iam also using it. however we facing problem with getting good and clean sequence.
Is it possible to share your...
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Forum: Sanger/Dye Terminator
11-19-2014, 08:11 PM
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Replies: 5
Views: 3,195
thanks guys
well my template is pol HIV gene...
thanks guys
well my template is pol HIV gene
this is kit based methods. I use Viroseq kit to test for resistance. I have used it in my training in Germany and now iam optimizing it here in our...
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Forum: Sanger/Dye Terminator
11-18-2014, 09:59 PM
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Replies: 5
Views: 3,195
thanks for reply sequencingfan
Well after...
thanks for reply sequencingfan
Well after receiving the analysis software we have seen that there are some setting we need to modify in analysis protocol we used.
We managed to get a quite good...
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Forum: Sanger/Dye Terminator
11-09-2014, 10:21 PM
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Replies: 5
Views: 3,195
low intensity signal with peaks under peaks
Hello
well iam doing sanger sequence for samples that used for resistance analysis. the bigdye chemistry is v1.1
after getting the sequence trace files raw data has shown low signal intensity...
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