Forum: Sample Prep / Library Generation
01-28-2020, 07:36 AM
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Replies: 0
Views: 1,106
1000 barcodes
Has anybody got any experience in pooling 1000 barcodes?
I am planning a nested multiplex PCR, testing several loci in 1000 clonal colonies for indels. Second PCR should introduce barcodes. IDT...
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Forum: Academic/Non-Profit Jobs
05-11-2017, 10:04 AM
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Replies: 0
Views: 1,179
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Forum: Sample Prep / Library Generation
06-16-2015, 07:59 AM
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Replies: 3
Views: 1,308
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Forum: Sample Prep / Library Generation
06-11-2015, 03:55 AM
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Replies: 3
Views: 1,308
problems with sample degradation in the lab
I have experienced some problems in the lab recently, and I am interested if anybody has any comment or suggestions.
We have noticed lower than usual yields for library preps. Now some of the data...
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Forum: Sample Prep / Library Generation
06-20-2013, 04:46 AM
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Replies: 48
Views: 31,891
I have not used Nextera at all, but I was...
I have not used Nextera at all, but I was intrigued by the comment that gDNA must be accurately quantified so that the tagmentation procedure works properly.
I have had a problem with accurately...
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Forum: Sample Prep / Library Generation
01-21-2013, 02:05 AM
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Replies: 12
Views: 8,147
Hello there, I would like to revive this thread...
Hello there, I would like to revive this thread because the same thing happened to us now as well. ChIP-Seq data shows no distinguishable peaks, but qPCR of both the sample and the library confirm...
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Forum: Sample Prep / Library Generation
07-17-2012, 05:00 AM
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Replies: 3
Views: 4,248
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Forum: RNA Sequencing
05-03-2012, 07:23 AM
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Replies: 1
Views: 1,900
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Forum: Sample Prep / Library Generation
03-06-2012, 03:51 AM
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Replies: 3
Views: 7,502
I have just run a test on our AMPure XP beads,...
I have just run a test on our AMPure XP beads, between new beads with ones that were 4 months over their expiry date. I compared different titrations on a ladder, and saw no difference whatsoever!
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Forum: Sample Prep / Library Generation
11-10-2011, 05:42 AM
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Replies: 2
Views: 1,990
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Forum: Sample Prep / Library Generation
11-03-2011, 09:24 AM
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Replies: 2
Views: 1,990
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Forum: Sample Prep / Library Generation
10-13-2011, 02:59 AM
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Replies: 5
Views: 12,282
Thanks everybody!
I guess the same applies to...
Thanks everybody!
I guess the same applies to the RNA-seq protocol, where having a large variety of molecules is even more important than having them all the same size.
Has anybody ever seen a...
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Forum: Sample Prep / Library Generation
10-06-2011, 08:49 AM
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Replies: 1
Views: 2,036
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Forum: Sample Prep / Library Generation
10-05-2011, 04:13 AM
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Replies: 1
Views: 2,036
Any advice on RNA bisulfite sequencing?
Hi everyone,
We are planning to treat total RNA (rRNA removed) with bisulfite and convert the nonmethylated cytosines to uracil. This is a harsh treatment and will brake up mRNA into very small...
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Forum: Sample Prep / Library Generation
10-04-2011, 02:27 AM
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Replies: 5
Views: 12,282
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Forum: Sample Prep / Library Generation
09-29-2011, 09:05 AM
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Replies: 5
Views: 12,282
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Forum: Illumina/Solexa
09-14-2011, 02:23 AM
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Replies: 5
Views: 2,897
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Forum: Core Facilities
12-08-2010, 02:24 AM
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Replies: 16
Views: 9,627
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Forum: Sample Prep / Library Generation
11-14-2010, 02:47 PM
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Replies: 5
Views: 5,878
Hello Gord,
Thanks for this insight. Would it...
Hello Gord,
Thanks for this insight. Would it be possible to pick your brains regarding the protocols for this endeavour? I am doubtful about several steps, eg shearing, the adapter ligation,...
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Forum: Sample Prep / Library Generation
11-11-2010, 06:57 AM
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Replies: 5
Views: 5,878
Thank you for the comments krobinson and...
Thank you for the comments krobinson and mrawlins! I think we are in a (long) queue for the PacBio machine, and the current strategy is RT at high temperatures. Fingers crossed...
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Forum: Sample Prep / Library Generation
11-09-2010, 06:23 AM
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Replies: 5
Views: 5,878
tRNA sequencing?
Hi,
I was wondering whether anybody has ever wanted to sequence tRNAs? I would be grateful for any information.
I am under the impression cDNA synthesis would be impaired by the modifications and...
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