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Search: Posts Made By: hanjaylee
Forum: Epigenetics 01-16-2010, 01:33 AM
Replies: 4
Views: 7,582
Posted By hanjaylee
hi. i am wondering if we are able to...

hi. i am wondering if we are able to deep-sequence the methylation pattern of the whole genome? i mean can we get the whole genome methylation state aside from the sequence information?
thanks a...
Forum: Sample Prep / Library Generation 01-15-2010, 04:32 PM
Replies: 15
Views: 5,170
Posted By hanjaylee
very much thanks, i will check it out.

very much thanks, i will check it out.
Forum: Sample Prep / Library Generation 01-14-2010, 03:18 PM
Replies: 15
Views: 5,170
Posted By hanjaylee
GW OK, THANKS FOR YOUR REPLY! would you...

GW OK,
THANKS FOR YOUR REPLY! would you please give me some more detailed information, as this is very important for me. thanks a million!!
Forum: Sample Prep / Library Generation 12-26-2009, 04:09 PM
Replies: 15
Views: 5,170
Posted By hanjaylee
thanks shurjo, i am wondering what you use to...

thanks shurjo,
i am wondering what you use to RT, oligo-dT or gene-specific primers, to obtaion your cdna product!
hanjay
Forum: Sample Prep / Library Generation 12-26-2009, 04:04 PM
Replies: 10
Views: 8,190
Posted By hanjaylee
Smile that is nice and meaningful! i will go check ...

that is nice and meaningful! i will go check the Roche protocol
Forum: Sample Prep / Library Generation 12-25-2009, 09:52 PM
Replies: 10
Views: 8,190
Posted By hanjaylee
Hi Phillip, i find you always posting very...

Hi Phillip,
i find you always posting very useful and constructive messages. i really appreciate that.
i want to ask if i want to construct a human genomic library with inserts ranging from 10kb to...
Forum: Sample Prep / Library Generation 12-25-2009, 06:06 PM
Replies: 27
Views: 10,049
Posted By hanjaylee
good challenge! i have been worrying about the...

good challenge! i have been worrying about the same problem, too.
Forum: Bioinformatics 12-25-2009, 05:40 PM
Replies: 236
Views: 523,602
Posted By hanjaylee
nice work!

nice work!
Forum: Sample Prep / Library Generation 12-25-2009, 04:15 PM
Replies: 15
Views: 5,170
Posted By hanjaylee
thanks very much, Phillip! i will check the...

thanks very much, Phillip! i will check the website. initially i was thinking of solexa, but it requires 5-10ug DNA, quite exceeding my sample.
Forum: Sample Prep / Library Generation 12-23-2009, 02:02 AM
Replies: 15
Views: 5,170
Posted By hanjaylee
the minimal amount of DNA required for deep-sequencing platforms!

hey. everyone! i have a sample containning many kinds of different cDNA, and i want to know their sequence and abundance in the sample. the problem is that the amount of the cDNA in the sample is...
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