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Forum: Illumina/Solexa 12-02-2015, 05:13 AM
Replies: 2
Views: 1,322
Posted By mikebravo
Hi Jim, we are using the biometra machines...

Hi Jim,

we are using the biometra machines (TPROFESSIONAL BASIC) to generate TS1 libraries and the are doing fine. You have to pay attention that your thermocycler is suitable for 100Ál. Otherwise...
Forum: Sanger/Dye Terminator 12-02-2015, 12:54 AM
Replies: 0
Views: 5,602
Posted By mikebravo
Randomly vanishing / extremely small G and C Peaks in Sequence

Hi all,

I want to ask if anyone ever experienced randomly small G and C peaks in their sequence. We are using BDT1.1 on a AB3130 and AB310 sequencer. Since a while we observe every here and than...
Forum: 454 Pyrosequencing 01-12-2011, 10:33 PM
Replies: 1
Views: 2,542
Posted By mikebravo
GS Junior - amplicon primers

Hi all,

we just got our GS Junior and I am currently designing primers for amplicon sequencing. Does anyone have some experience with it? For example which amplicon size is acceptable for...
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