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Search: Posts Made By: GA-J
Forum: Illumina/Solexa 12-07-2017, 07:24 PM
Replies: 15
Views: 3,259
Posted By GA-J
For Miseq, whatever you started, you may adjust...

For Miseq, whatever you started, you may adjust to load 10pM. I have done this a few times, it worked well. You don't have to spike more than 5%, I used 1% spike.
Forum: Illumina/Solexa 12-07-2017, 06:14 PM
Replies: 15
Views: 3,259
Posted By GA-J
You may pool equal mol for each sample, then find...

You may pool equal mol for each sample, then find the final pooled lib con. Meanwhile, if you receive 0.5nM, look at the protocol, you could do as below: Starting Library Concentration Library 0.2 N...
Forum: Illumina/Solexa 11-08-2017, 07:37 PM
Replies: 15
Views: 3,259
Posted By GA-J
A and C looks good to me, D looks over-fragmented...

A and C looks good to me, D looks over-fragmented due to low starting mass.

B ? The size looks too large.

But I think you will have good data yield for A,C and D, may receive less data from...
Forum: Sample Prep / Library Generation 04-05-2017, 06:19 PM
Replies: 3
Views: 5,266
Posted By GA-J
It will not affect your seq, or limited affect....

It will not affect your seq, or limited affect. It may be a dimer of your product.
Forum: Sample Prep / Library Generation 04-05-2017, 06:12 PM
Replies: 5
Views: 1,842
Posted By GA-J
Actually I think it may come from 18s or 28s if...

Actually I think it may come from 18s or 28s if your starting mass was too high.I saw some peaks from mRNA lib that started from poor quality of RNA. Not for sure, just a guess.
Forum: Illumina/Solexa 12-07-2016, 07:08 PM
Replies: 17
Views: 10,483
Posted By GA-J
NextSeq kit close to expire date

Does anybody know the different between NextSeq High output 300cycles and 150cycles kit? Only the reagent volume different? May I combine 2 of 150cycles kit for a 300 cycles run?
Forum: Metagenomics 10-11-2016, 06:32 PM
Replies: 4
Views: 2,139
Posted By GA-J
Does the training fee include the...

Does the training fee include the stay(hotel+food)? I may want to attend with my own cost. Thanks for your information.
Forum: Metagenomics 10-11-2016, 06:22 PM
Replies: 4
Views: 2,139
Posted By GA-J
I am interested.Have to be on site? A little far...

I am interested.Have to be on site? A little far away.
Forum: Illumina/Solexa 12-01-2015, 11:31 AM
Replies: 2
Views: 1,424
Posted By GA-J
Back order!

Yes, same here! I am out of cartridges. Anyone around Atlanta GA may help me out??
Forum: Illumina/Solexa 11-19-2015, 12:27 PM
Replies: 5
Views: 2,753
Posted By GA-J
Ask about spacer-primers. It may help you out.

Ask about spacer-primers. It may help you out.
Forum: Illumina/Solexa 10-28-2015, 07:17 PM
Replies: 24
Views: 9,157
Posted By GA-J
I would like to share some information from the...

I would like to share some information from the stopped run. Thanks for any words.

Newest update: the rerun passed without error! Quality looks OK.
Forum: Illumina/Solexa 10-28-2015, 11:54 AM
Replies: 24
Views: 9,157
Posted By GA-J
Thank you very much! I will try to work it out!:)

Thank you very much! I will try to work it out!:)
Forum: Illumina/Solexa 10-28-2015, 08:46 AM
Replies: 24
Views: 9,157
Posted By GA-J
Yes,we got replacement kit. My problem is there...

Yes,we got replacement kit. My problem is there is no name appear at my Miseq reporter, how can I work it out?
Thank you!
Forum: Illumina/Solexa 10-27-2015, 06:08 PM
Replies: 24
Views: 9,157
Posted By GA-J
I had a stopped miseq run, 2x300, it stopped at...

I had a stopped miseq run, 2x300, it stopped at 546 cycle. Run completed with error message: best focus is too near edge to the range.So no fastq files generated. Anyone knows what cause this...
Forum: Sample Prep / Library Generation 10-09-2015, 06:40 PM
Replies: 5
Views: 3,020
Posted By GA-J
I would just use this library(0.7nM), denature...

I would just use this library(0.7nM), denature with 0.1N NaoH, then it comes to 7pM after 100x dilution with HT1 buffter. If your library's size is about 400bp, you may load at this concentration or...
Forum: Illumina/Solexa 09-25-2015, 07:48 PM
Replies: 7
Views: 4,321
Posted By GA-J
@nucacidhunter: Thank you for your opinion. I am...

@nucacidhunter: Thank you for your opinion. I am not able to post a screenshot now. I could try on next Monday. But there is no reason to be overloaded. Let's try to dig more.:)
Forum: Illumina/Solexa 09-25-2015, 04:45 PM
Replies: 7
Views: 4,321
Posted By GA-J
Recently I had a bad run with overcluster...

Recently I had a bad run with overcluster density(over 1100 for V2), but we saw very very low passing filter and very low phix. For troubleshooting, we checked everything, libraries no problem, prep...
Forum: Illumina/Solexa 08-27-2015, 08:14 AM
Replies: 10
Views: 9,465
Posted By GA-J
Thank you, Jessica L, sounds very clear and...

Thank you, Jessica L, sounds very clear and simple to follow.
Forum: Illumina/Solexa 08-26-2015, 10:53 AM
Replies: 10
Views: 9,465
Posted By GA-J
Miseq wash question

Does anyone know where I may find miseq wash record from miseq computer? Any folder or file? Thanks in advance.:)

Another one: if I have next run maybe in 2 or 3 weeks, I don't want to do stand-by...
Forum: Illumina/Solexa 08-10-2015, 07:13 PM
Replies: 3
Views: 1,209
Posted By GA-J
I would simply test a few primers with index-PCR,...

I would simply test a few primers with index-PCR, and run on BA side by side.
Forum: Metagenomics 08-03-2015, 09:09 AM
Replies: 19
Views: 7,381
Posted By GA-J
Thank you, Fanli.

Thank you, Fanli.
Forum: Metagenomics 08-03-2015, 07:05 AM
Replies: 19
Views: 7,381
Posted By GA-J
Fanli, thank you for your information. Two more...

Fanli, thank you for your information. Two more questions, did you use Miseq V2 kit for this 16s V4 run? Why no Phix spike in(how do you decide no Phix, any protocol mentioned or you tested it out?...
Forum: Metagenomics 07-24-2015, 07:43 PM
Replies: 19
Views: 7,381
Posted By GA-J
Hello, Fanli, I like your result of 16s V4 Miseq...

Hello, Fanli, I like your result of 16s V4 Miseq run. I would like to know how much you loaded? And the size of your library is ?

Thanks.:)
Forum: Metagenomics 07-24-2015, 07:38 PM
Replies: 5
Views: 2,314
Posted By GA-J
Hello, Fanli, I like your result of 16s V4 Miseq...

Hello, Fanli, I like your result of 16s V4 Miseq run. I would like to know how much you loaded? And the size of your library is ?

Thanks.:)
Forum: Illumina/Solexa 07-20-2015, 12:25 PM
Replies: 10
Views: 3,265
Posted By GA-J
Fantastic! I will work on it, thank you again,...

Fantastic! I will work on it, thank you again, Microgirl123 !:)
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