SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 25 of 65
Search took 0.00 seconds.
Search: Posts Made By: Chuckytah
Forum: Bioinformatics 02-05-2013, 06:34 AM
Replies: 0
Views: 844
Posted By Chuckytah
Lightbulb transcriptome assembly with reference

Hello, me and some professors wanted programs that align contigs to a reference transcriptome. We would like user-friendly programs and preferentially for Windows. What is the best pipeline? thanks...
Forum: Bioinformatics 10-24-2012, 02:55 PM
Replies: 1
Views: 2,149
Posted By Chuckytah
Still did not discover anything :( The link...

Still did not discover anything :(

The link above is not working, try this one:
http://telethon.bio.unipd.it/bioinfo/IDEG6_form/detail.html
Forum: General 09-21-2012, 11:57 AM
Replies: 1
Views: 877
Posted By Chuckytah
ammonium is related to anatomical structure...

ammonium is related to anatomical structure morphogenesis?
Forum: General 09-21-2012, 09:50 AM
Replies: 1
Views: 877
Posted By Chuckytah
Plants vs mycorrhizas

Why plants ammonium transporter is underexpressed in mycorrhizal samples? :confused:
Forum: Bioinformatics 07-16-2012, 03:51 AM
Replies: 2
Views: 2,170
Posted By Chuckytah
I really can't remember if I did any alteration...

I really can't remember if I did any alteration in the parameters. lol but I already solve my problem in tab Tools there is an option that "Restore all original B2G properties", and now everything is...
Forum: Bioinformatics 07-13-2012, 05:42 AM
Replies: 2
Views: 2,170
Posted By Chuckytah
Angry From Fasta get GO-terms

Hello friends,

I used to do GO annotation using Blast2GO but it strangely gave me 0 GO-terms to a 507 sequences dataset... it was really strange... The blast went fine, but when I try the...
Forum: Bioinformatics 07-12-2012, 12:26 PM
Replies: 1
Views: 2,149
Posted By Chuckytah
IDEG6 - Normalization question - Identification Differentially Expressed Genes

Hello friends,

I'm using IDEG6 for Identification Differentially Expressed Genes but they normalize my values and I don't know how they do that... anyone knows?
:confused:
...
Forum: Bioinformatics 07-05-2012, 01:15 PM
Replies: 1
Views: 1,798
Posted By Chuckytah
Unhappy Blast2GO problem with .dat saved files

I have a .dat file that when I open it in blast2go it has 50.000 sequences... i run mapping, and in midle of the run I save the .dat file... then I close blast2go and next time I wanna open the more...
Forum: RNA Sequencing 07-04-2012, 02:16 PM
Replies: 2
Views: 1,546
Posted By Chuckytah
Thanks so much for the answer. The mRNA came only...

Thanks so much for the answer. The mRNA came only from root sample, but there were always contaminants. My concern now it's to discover why the variation in alpha diversity... not the genes ;) but...
Forum: Academic/Non-Profit Jobs 07-04-2012, 12:36 PM
Replies: 0
Views: 1,503
Posted By Chuckytah
Red face (Scotland) - Internship Position under the Leonardo da Vinci program

Dear Sir/Madam,

I would like to know about the possibility in doing an internship in your company/research group.
I'm graduated in Biology-Geology and soon will finish my master in...
Forum: RNA Sequencing 07-04-2012, 12:29 PM
Replies: 2
Views: 1,546
Posted By Chuckytah
Question Water stress and Mycorrhization - Alpha diversity conclusions??

Hello all,

first of all I will made a brief resume about my thesis::confused:
I had 2 sub-projects, project A - abiotic stress (water stress) and project M - biotic stress (mycorrhization). I...
Forum: General 06-01-2012, 05:16 PM
Replies: 3
Views: 2,644
Posted By Chuckytah
No other suggestions?:confused:

No other suggestions?:confused:
Forum: General 05-14-2012, 11:43 AM
Replies: 3
Views: 2,644
Posted By Chuckytah
Yes, i'm contacting universities... but would be...

Yes, i'm contacting universities... but would be nice to have some kind of list of companies/enterprises...
Forum: General 05-13-2012, 05:22 AM
Replies: 3
Views: 2,644
Posted By Chuckytah
request for advice - graduated in Biology-Geology, master in Bioinformatics

Good Afternoon,

first of all I apologize for the "invasion" and to request your time for me. I'm a student of Masters in Bioinformatics at the University of Minho, Portugal, that soon will end my...
Forum: Bioinformatics 03-15-2012, 12:51 PM
Replies: 9
Views: 3,377
Posted By Chuckytah
still too small, i choose 0.06 and the words are...

still too small, i choose 0.06 and the words are above other/on top again... and if I choose 0.03 for example, when I save to .pdf the words dont apear at all :S
Forum: Bioinformatics 03-15-2012, 10:03 AM
Replies: 9
Views: 3,377
Posted By Chuckytah
changing the cexRow did not worked... :S cant...

changing the cexRow did not worked... :S cant read at all ... :S :(
Forum: Bioinformatics 03-15-2012, 09:26 AM
Replies: 9
Views: 3,377
Posted By Chuckytah
Already here my R codes :)

Already here my R codes :)
Forum: Bioinformatics 03-15-2012, 09:25 AM
Replies: 9
Views: 3,377
Posted By Chuckytah
I've put 0.5... you advise me to put what? :)

I've put 0.5... you advise me to put what? :)
Forum: Bioinformatics 03-15-2012, 09:25 AM
Replies: 9
Views: 3,377
Posted By Chuckytah
genes <- read.csv("genes.csv", sep=",") ...

genes <- read.csv("genes.csv", sep=",")
row.names(genes) <- genes$Descritpion
genes <- genes[,2:4]
genes_matrix <- data.matrix(genes)
genes_heatmap <- heatmap(genes_matrix, Rowv=NA, Colv=NA,col =...
Forum: Bioinformatics 03-15-2012, 08:59 AM
Replies: 9
Views: 3,377
Posted By Chuckytah
I will try those suggestions. and i will post my...

I will try those suggestions. and i will post my script here :) thanks for answers
Forum: Bioinformatics 03-14-2012, 11:38 AM
Replies: 9
Views: 3,377
Posted By Chuckytah
Question R - problem in heatmap reading

Hi all, i'm using R to draw heatmaps... but I have a huge amount of data, around 600 genes... Did anyone could suggest me a better way to draw it? this image is to show you that the gene descritption...
Forum: 454 Pyrosequencing 03-05-2012, 11:52 AM
Replies: 0
Views: 1,341
Posted By Chuckytah
Unhappy Blastclust 85%???

Hello

i'm sorry if my question will be very stupid... but i'm lost =x

I have my data something like this
Genes Seq.Length Number EST Blastclust 85%
gene A 600 9 ...
Forum: Introductions 03-04-2012, 11:28 AM
Replies: 373
Views: 141,154
Posted By Chuckytah
It is a transcritpomic analysis but i need DGE...

It is a transcritpomic analysis but i need DGE too.
Forum: Introductions 03-04-2012, 03:11 AM
Replies: 373
Views: 141,154
Posted By Chuckytah
Welcome! I'm working with NGS too but for my...

Welcome! I'm working with NGS too but for my thesis... i need help for differential gene expression... i'm new to this.
Forum: Bioinformatics 03-03-2012, 09:27 AM
Replies: 7
Views: 7,833
Posted By Chuckytah
In the topic: Next generation sequencing for gene...

In the topic: Next generation sequencing for gene expression measurement

i'm doing RA (relative abundances)... I have the number of EST in one gene and i divide it by the total number of EST in...
Showing results 1 to 25 of 65

 


All times are GMT -8. The time now is 10:28 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO