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Forum: RNA Sequencing 02-18-2019, 05:53 PM
Replies: 2
Views: 1,100
Posted By fanzhen
The following is my command. tophat2 -p 8 -r...

The following is my command.
tophat2 -p 8 -r 300 -N 1 -o tophat_out /path/ref read1 read2
And I have ref.fa and Bowtie built reference in this folder.
The last line in log file is 'Generating SAM...
Forum: RNA Sequencing 02-18-2019, 02:03 PM
Replies: 2
Views: 1,100
Posted By fanzhen
Tophat error

I am new to RNAseq, I am trying to map my reads to my reference genome. It generates this error
Generating SAM header for /path/ref
Traceback (most recent call last):
File...
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