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Forum: Sample Prep / Library Generation 03-24-2016, 01:20 AM
Replies: 69
Views: 43,569
Posted By mantis
Hi puraskar theaward, To answer your...

Hi puraskar theaward,

To answer your questions:
1.1 Yes, I used the full range. So, my average size was around 450-500 for each sample; I calculated the molarity from that to obtain eventually 4...
Forum: Sample Prep / Library Generation 03-08-2016, 01:51 AM
Replies: 69
Views: 43,569
Posted By mantis
I never did...

I never did...
Forum: Sample Prep / Library Generation 03-01-2016, 12:21 AM
Replies: 69
Views: 43,569
Posted By mantis
Hi Runuply, Yes, I did a 1.6V ampure...

Hi Runuply,

Yes, I did a 1.6V ampure treatment: The small primers won't bind to the peaks with this DNA/beads ratio.

Good luck!
Forum: Sample Prep / Library Generation 02-29-2016, 12:28 AM
Replies: 69
Views: 43,569
Posted By mantis
Looks good Runuply: there seems to be a good...

Looks good Runuply: there seems to be a good nucleosome pattern on the gel picture. I think I would advice you to try to get rid of the primer peak at 65 before submitting the sample for sequencing.
Forum: Sample Prep / Library Generation 02-26-2016, 08:14 AM
Replies: 69
Views: 43,569
Posted By mantis
Gel of good ATAC-seq experiment

hi everyone,

I just tried my first ATAC-seq on cells and using the protocol of Buenrostro and I obtain good results: sharp peaks, low background, (appr. 50000 cells).

I included my HS picture....
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