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Forum: General 10-17-2016, 10:44 PM
Replies: 6
Views: 3,104
Posted By Sylv
HI, and thanks for you input. I thought of...

HI, and thanks for you input.
I thought of that, but the same buffer has been used several times perviously without causing trouble. It was also done the same procedure on DNA samples isolated by...
Forum: General 10-17-2016, 10:37 PM
Replies: 6
Views: 3,104
Posted By Sylv
Hi, and thanks for you input. I also thought of...

Hi, and thanks for you input.
I also thought of this, as Illumina protocol has very long drying of the beads. But I tried drying them for a shorter time, and it made no difference.
Forum: General 10-17-2016, 01:20 AM
Replies: 6
Views: 3,104
Posted By Sylv
Thank you for your reply. The beads does not...

Thank you for your reply. The beads does not clump together as such when adding them to the DNA, they just stick to the wells of the plate. The quality of the DNA was checked using the NanoDrop...
Forum: Sample Prep / Library Generation 10-13-2016, 10:31 PM
Replies: 4
Views: 2,283
Posted By Sylv
We start out with 500ng DNA in 35Ál, and add 15Ál...

We start out with 500ng DNA in 35Ál, and add 15Ál restriction mastermix so a total of 50Ál. 75Ál beads are then used for purification.
The beads are dried for 5 min. Illumina protocol says at least...
Forum: Sample Prep / Library Generation 10-13-2016, 04:41 AM
Replies: 4
Views: 2,283
Posted By Sylv
Problems using Agencourt AmPure XP beads.

Hi.
I am testing some DNA samples for doing ddRAD sequencing, and I have a problem.
After restriction digest, I purify the reaction using Agencourt Ampure XP beads. When eluting the DNA in water,...
Forum: General 10-13-2016, 04:02 AM
Replies: 6
Views: 3,104
Posted By Sylv
Agencourt Ampure XP beads

Hi.
I am testing some DNA samples for doing ddRAD sequencing, and I have a problem.
After restriction digest, I purify the reaction using Agencourt Ampure XP beads. When eluting the DNA in water,...
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