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Forum: Illumina/Solexa 11-11-2016, 03:19 AM
Replies: 1
Views: 1,438
Posted By chicken
Adapter titration in TruSeq stranded mRNA sample preparation

Hi,
I am doing transcriptome analysis and prepared some RNA libraries from total RNA. Allways using 500ng of total RNA. I always got the problem that I got a lot of adapter dimers in the end. I...
Forum: Illumina/Solexa 11-11-2016, 03:10 AM
Replies: 4
Views: 1,132
Posted By chicken
Hey thanks for your suggestions. Now I am quite...

Hey thanks for your suggestions. Now I am quite sure that the peaks show adapter dimers. Unfortunately my kit manual does not show the option to dilute the adapter. Is this not common in illumina...
Forum: Illumina/Solexa 09-08-2016, 01:57 AM
Replies: 4
Views: 1,132
Posted By chicken
only short fragments (90, 124bp) after amplification

Dear community,

I am new at RNA seq with Illumina and hope you can help me.
We use the MiSeq System with the TruSeq Stranded mRNA Sample Prep Kit. I do low throughput.
After the amplification...
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