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Forum: Ion Torrent 07-23-2015, 09:59 AM
Replies: 2
Views: 1,394
Posted By ptery
I use 70ul @ 100pM for the old IC kit. For Hi-Q...

I use 70ul @ 100pM for the old IC kit. For Hi-Q kit, I first followed the protocol with 25ul @ 50pM with only 49% loading. So I increased loading to 25ul @ 200pM, the result is still not good....
Forum: Ion Torrent 07-23-2015, 08:15 AM
Replies: 2
Views: 1,394
Posted By ptery
Problem with new Hi-Q Chef kit for proton

With the discontinue of the old PI IC kit, we have to switch to new Hi-Q chef kit. We had great success with the old PI IC kit, but facing dramatic drop in loading percentage and read quality with...
Forum: General 06-17-2015, 10:56 AM
Replies: 2
Views: 1,508
Posted By ptery
Help with size selection

I want to remove a 33nt RNA from a small quantity pool of total RNA (couple hundred ng). What kind of technique/kits should I use? Does Ampure XP beads work for RNA? or there is another beads for RNA...
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