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Forum: Illumina/Solexa 04-09-2010, 12:36 PM
Replies: 32
Views: 21,772
Posted By mccullou
Talking ECO- I was thinking the same thing :rolleyes: ...

ECO- I was thinking the same thing :rolleyes:

For those that have the SPRI, perhaps I am missing something in the literature, but does this instrumentation do the complete library prep from...
Forum: Illumina/Solexa 03-26-2010, 11:57 AM
Replies: 21
Views: 13,687
Posted By mccullou
I agree with peromhc. And I will add, even when...

I agree with peromhc. And I will add, even when optimized it seems the reaction is VERY sensitive to any changes. One sample will display a good smear another seemingly no digestion. We stayed...
Forum: Illumina/Solexa 03-26-2010, 11:52 AM
Replies: 9
Views: 4,460
Posted By mccullou
Same here

Looking for Library prep automation to increase throughput and eliminate variations caused by different users. In addition to the automation mentioned above from BIG_SNP, are there any other new...
Forum: Illumina/Solexa 01-27-2010, 08:39 AM
Replies: 73
Views: 351,126
Posted By mccullou
So are most buffers if you ask qiagen or...

So are most buffers if you ask qiagen or invitrogen, but we are all smart people here, right? It is either photo or chemical cleavage and looking at all the steps in the sequencing process there...
Forum: Illumina/Solexa 01-13-2010, 08:42 AM
Replies: 14
Views: 15,930
Posted By mccullou
Well that is good and bad. Good to see that the...

Well that is good and bad. Good to see that the biochemistry is rather unchanged (aside from repackaging reagents for HiSeq use only). That way hopefully upstream modifications made for specific...
Forum: Illumina/Solexa 01-12-2010, 07:27 PM
Replies: 14
Views: 15,930
Posted By mccullou
Illumina's new sequencer: HiSeq 2000

Wow GAII just came out last year, they just now got the reagents QC under control after their SNAFU and now this. . .

http://www.illumina.com/systems/hiseq_2000.ilmn

How different can this...
Forum: Illumina/Solexa 12-10-2009, 01:53 PM
Replies: 1
Views: 5,737
Posted By mccullou
Minimum size of DNA insertion fragments

I have small DNA fragments (avg 44 nt) that I would like to sequence but this is out of the recommended range of the GAII. I understand the limits of larger fragments (limited cluster density/large...
Forum: General 12-08-2008, 09:33 AM
Replies: 0
Views: 1,972
Posted By mccullou
Fluidigm can help with sequencing?

I have read in various places (mostly their literature) that Fluidigm "eliminates costly titration steps" in next generation sequencing. I am unclear exactly how their technology really helps. Are...
Forum: The Pipeline 10-22-2008, 10:05 AM
Replies: 0
Views: 8,300
Posted By mccullou
Oxford Nanopore

For originality this is the first 3rd gene sequencing by destruction. An exonuclease feeds cleaved bases into a modified hemolysin, while the potential is monitored. According to Oxford modified...
Forum: The Pipeline 10-22-2008, 08:40 AM
Replies: 0
Views: 2,939
Posted By mccullou
NabSys 3rd gen

NabSys uses nanopores, combined with positional information obtained from sequential hybridization of a library of hexamers, to build sequence.
http://www.nabsys.com/
Forum: The Pipeline 10-22-2008, 08:29 AM
Replies: 1
Views: 5,518
Posted By mccullou
Another company with deep pockets ponies up for...

Another company with deep pockets ponies up for 3rd gen. This looks like a hybrid between PacBio (single molecule, flurophore on the PO4, monitor incorporation) and Helicos (oligo array, visualize,...
Forum: Introductions 10-21-2008, 12:06 PM
Replies: 0
Views: 1,069
Posted By mccullou
Hello All

I am a scientist working on 3rd gen nanopore sequencing technology. I work on mostly the upstream biochemistry, but as of late I have been dealing with novel technologies, instrumentation, and IP as...
Forum: Illumina/Solexa 10-16-2008, 09:05 AM
Replies: 73
Views: 351,126
Posted By mccullou
Fluorophore removal

How does Illumina remove the fluorophore? They can't use AgNO3 (like SOLiD ) because of no Thio backbone. Are they using the Columbia IP that Intelligent BioSystems is using? ...
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