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Search: Posts Made By: lior lobel
Forum: Bioinformatics 01-21-2014, 06:27 AM
Replies: 0
Views: 867
Posted By lior lobel
ChIP Seq analysis of bacterial samples

Hi All,

Has anyone done a ChIP-seq analysis of bacterial samples?
What tools would you recommend using? (e.g. MACS) are there any tools tailored for prokaryotes?

Thanks,

Lior
Forum: Sample Prep / Library Generation 07-17-2013, 08:14 AM
Replies: 1
Views: 3,421
Posted By lior lobel
rRNA sizes on Agarose gel

Hi All,

I ran a Agarsoe gel with my RNA samples and got the attached pattern.
23S rRNA and 16S rRNA are supposed to be ~3000 bps and ~1500 bps, however in this run I see 1500 and 750 bands.
...
Forum: Sample Prep / Library Generation 07-05-2013, 06:20 AM
Replies: 4
Views: 1,818
Posted By lior lobel
Hi Helena, Just yesterday did the...

Hi Helena,

Just yesterday did the bioanalyzer analysis. please see attached. This is after rRNA depletion by RiboZero and by mistake the analysis was done with Eukaryotic reference.

The big...
Forum: Sample Prep / Library Generation 06-30-2013, 07:38 AM
Replies: 4
Views: 1,818
Posted By lior lobel
Question Help with determining RNA quality for RNA SEQ

Hi All,

I'm new to RNA-Seq, so I'm not that familier with RNA quality checks for RNA seq. I have 6 total bacterial RNA samples, that I want to carry for rRNA depletion using the RiboZero kit....
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