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Search: Posts Made By: lyndaben
Forum: Epigenetics 09-29-2015, 10:10 AM
Replies: 26
Views: 10,919
Posted By lyndaben
If you figure it out, please would you post the...

If you figure it out, please would you post the solution? We are prepping libraries with the new kit at the moment, so it would be good to know before we have them sequenced :)
Forum: Epigenetics 09-29-2015, 10:00 AM
Replies: 26
Views: 10,919
Posted By lyndaben
Faria, did you use the old kit or the new kit?

Faria, did you use the old kit or the new kit?
Forum: Epigenetics 09-29-2015, 07:32 AM
Replies: 26
Views: 10,919
Posted By lyndaben
We used the NuGEN Ovation kit with great results...

We used the NuGEN Ovation kit with great results when we multiplexed our samples with RNA-seq samples. When we multiplexed some RRBS libraries with PhiX we had problems with demultiplexing but when...
Forum: Epigenetics 07-07-2015, 11:35 AM
Replies: 5
Views: 3,281
Posted By lyndaben
We used the NEBNext Methylated adapters and we...

We used the NEBNext Methylated adapters and we got what I thought were pretty reasonable libraries. We do however have large peaks at 186-188bp in some of the libraries. Are these adapter dimers? ...
Forum: Epigenetics 03-13-2015, 07:59 AM
Replies: 26
Views: 10,919
Posted By lyndaben
The new kit will be better hopefully. A note of...

The new kit will be better hopefully. A note of caution to anyone using the old kit with the modified RRBS protocol: The first sequencing run we multiplexed with RNAseq samples and everything was...
Forum: Epigenetics 03-10-2015, 12:55 PM
Replies: 5
Views: 3,281
Posted By lyndaben
I am about to try the TrueMethyl kit for the...

I am about to try the TrueMethyl kit for the first time (for RRBS). Did you find a library prep protocol that worked for you? I don't think Illumina sell methylated adapters. I did find the...
Forum: Epigenetics 07-24-2014, 09:09 AM
Replies: 26
Views: 10,919
Posted By lyndaben
I opted for the NuGEN Ovation kit. They provided...

I opted for the NuGEN Ovation kit. They provided me with a protocol addition for RRBS. It was just delivered this week so I have not had the chance to try it yet
Forum: Epigenetics 06-05-2014, 03:10 PM
Replies: 26
Views: 10,919
Posted By lyndaben
which kit for RRBS?

Any advice on which kit to use for RRBS? I'm looking at Epignome Methyl-Seq; NuGen Ovation Ultra low Methyl-seq; Bioo NEXTflex bisulfite sequencing kit,
The Illumina web site is confusing - their...
Forum: Sample Prep / Library Generation 06-05-2014, 02:46 PM
Replies: 3
Views: 1,708
Posted By lyndaben
Has anyone out there used the NEXTflex kit for...

Has anyone out there used the NEXTflex kit for RRBS?
Forum: RNA Sequencing 05-14-2013, 03:36 PM
Replies: 7
Views: 5,226
Posted By lyndaben
We are seeing a big difference according to the...

We are seeing a big difference according to the method of cell lysate storage and RNA isolation: With Qiazol and miRNeasy-performing RNA-Seq we get lots of intronic reads that looks very much like...
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