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Forum: Sample Prep / Library Generation 02-09-2015, 01:00 AM
Replies: 9
Views: 6,676
Posted By Babooncanfly
Awesome thanks Simone!

Awesome thanks Simone!
Forum: Sample Prep / Library Generation 02-09-2015, 12:30 AM
Replies: 9
Views: 6,676
Posted By Babooncanfly
Thanks Simone. I posted a similar message in...

Thanks Simone. I posted a similar message in another thread but thanks for answering here. What do you recommend that we do, just exclude humanin from future analysis? You still stand by the rest of...
Forum: Sample Prep / Library Generation 02-08-2015, 07:19 PM
Replies: 270
Views: 112,356
Posted By Babooncanfly
Hi Simone it may be helpful to know that I saw...

Hi Simone it may be helpful to know that I saw this 1.85kb peak from bulk purified RNA as well as single cells, so it's unlikely stress on the cell. Furthermore, we don't see this with Smart-seq1,...
Forum: Sample Prep / Library Generation 02-08-2015, 06:38 PM
Replies: 9
Views: 6,676
Posted By Babooncanfly
18s peak?

Hi op I'm wondering if you looked at cDNA size distribution by bioanalyzer, and saw any spike at 1.85kb? We saw this consistently, which is absent from Smarts-seq 1 protocol. Thanks in advance.
Forum: Bioinformatics 03-05-2013, 12:20 PM
Replies: 0
Views: 1,238
Posted By Babooncanfly
Question hsteq-count giving zero reads on abundant gene

Dear all and Simon,

htseq-count ran fine on my mm9_transcriptome gtf file. No warning messages.

The output .count file looks mostly correct also. However, an abundant gene "Terc" gives zero...
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