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Forum: Epigenetics 06-21-2011, 05:37 PM
Replies: 4
Views: 2,832
Posted By Theorbe27
Hi mudshark, I am not entirely sure about...

Hi mudshark,

I am not entirely sure about all aligner parameters since I have only received aligned reads from our seq facility. I would have to ask them for more details if this turns out to be...
Forum: Epigenetics 06-20-2011, 02:01 PM
Replies: 4
Views: 2,832
Posted By Theorbe27
Question ChIPseq on tissue - eliminating background

Hi,

I am trying to do ChIPseq on tissue for a transcription factor that is expressed in a single cell type within a tissue. Therefore, specific signal is only going to be derived from about 3% of...
Forum: Sample Prep / Library Generation 02-18-2011, 01:18 PM
Replies: 2
Views: 3,407
Posted By Theorbe27
Question ChipSeq library construction from tissue and DNA purity issues

Hi,

we are currently trying to construct a ChIP-Seq library from a tissue sample. To complicate things, the transcription factor we would like to assay for is only expressed in a fraction (maybe...
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