SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 5 of 5
Search took 0.04 seconds.
Search: Posts Made By: jwfoley
Forum: Sample Prep / Library Generation 04-17-2015, 12:41 PM
Replies: 15
Views: 16,447
Posted By jwfoley
We now have a protocol for RNA beads (comparable...

We now have a protocol for RNA beads (comparable to RNAClean XP) as well. I've added it to the original post.
Forum: Sample Prep / Library Generation 02-09-2015, 07:42 AM
Replies: 15
Views: 16,447
Posted By jwfoley
Yes, actually, we had the same problem but I...

Yes, actually, we had the same problem but I thought it was just a temporary stock issue. Some of the websites will actually tell you whether they're in stock before you order.

At least once you...
Forum: Sample Prep / Library Generation 01-27-2015, 05:12 AM
Replies: 15
Views: 16,447
Posted By jwfoley
No, that sounds right; 100 mL of the Sera-Mag...

No, that sounds right; 100 mL of the Sera-Mag beads will make 5 L of SPRI mix, and that much AMPure XP would cost about $72,000 CAD at our price (though we could probably get a volume discount). Are...
Forum: Sample Prep / Library Generation 01-14-2015, 06:24 AM
Replies: 15
Views: 16,447
Posted By jwfoley
Have you found that Rohland and Reich's recipe...

Have you found that Rohland and Reich's recipe works at low volume ratios (0.7X or 0.6X)? It has a slightly lower PEG concentration and especially a much lower NaCl concentration, so I'd worry that...
Forum: Sample Prep / Library Generation 01-13-2015, 06:56 AM
Replies: 15
Views: 16,447
Posted By jwfoley
Make your own AMPure XP, cheaply

Like a lot of labs, we've replaced spin/vacuum columns with magnetic bead extraction for every part of our library construction protocols. However, the commercial products are expensive. We found...
Showing results 1 to 5 of 5

 


All times are GMT -8. The time now is 12:05 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2022, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO