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Forum: RNA Sequencing 02-12-2014, 11:32 AM
Replies: 2
Views: 1,836
Posted By dbroh11
Experimental Design Given One Cell Line

We are interested in examining the effects of four different drug compounds (with 1 being a "control" in the form of a growth factor) on differential gene expression in a motor neuron cell line.
...
Forum: Sample Prep / Library Generation 02-12-2014, 10:23 AM
Replies: 5
Views: 2,385
Posted By dbroh11
I know this post is fairly old, but in a similar...

I know this post is fairly old, but in a similar vein, we are interested in examining the effects of four different drug compounds (with 1 being a "control" in the form of a growth factor) on...
Forum: RNA Sequencing 02-11-2014, 05:08 PM
Replies: 0
Views: 1,335
Posted By dbroh11
RNA-Seq Statistics

Can anyone recommend a good book, website, literature review etc. that can help me educate myself about the statistics behind RNA-Seq analysis?
Forum: RNA Sequencing 02-03-2014, 09:02 AM
Replies: 11
Views: 4,661
Posted By dbroh11
I appreciate your guys help with this - Do you...

I appreciate your guys help with this - Do you have literature aside from the paper GenoMax sent that supports using far less than 100M reads (what Encode proposed?) I understand ENCODE is not the...
Forum: RNA Sequencing 01-31-2014, 10:45 AM
Replies: 11
Views: 4,661
Posted By dbroh11
Hey Guys, It looks like for a run of the...

Hey Guys,

It looks like for a run of the mill differential gene expression analysis, 20-30 M reads is more than sufficient based on your response, Tony, and the paper that GenoMax kindly supplied....
Forum: Introductions 01-31-2014, 07:32 AM
Replies: 3
Views: 1,674
Posted By dbroh11
Yup - didn't know how to change forums when I...

Yup - didn't know how to change forums when I first signed up - Thank you for your help!
Forum: RNA Sequencing 01-31-2014, 07:19 AM
Replies: 11
Views: 4,661
Posted By dbroh11
RNA-Seq Experimental Design Questions

Hello,

My name is David Brohawn and I am new to RNA-Seq.

My advisor and I are interested in doing an RNA-Seq experiment to compare the transcriptomes of iPSC neurons we generate from both ALS...
Forum: Introductions 01-31-2014, 07:00 AM
Replies: 3
Views: 1,674
Posted By dbroh11
RNA-Seq Experimental Design

Hello,

My name is David Brohawn and I am new to RNA-Seq.

My advisor and I are interested in doing an RNA-Seq experiment to compare the transcriptomes of iPSC neurons we generate from both ALS...
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