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Forum: Sample Prep / Library Generation 09-25-2019, 02:48 AM
Replies: 0
Views: 1,429
Posted By provirus
Tapestation imaging

How does the Tapestation (2200/4200) measure fluorescently stained biomolecules ?
Does it scan fluorescence in real-time (during eletrophoresis) at a single position or after eletrophoresis has...
Forum: Illumina/Solexa 10-18-2017, 01:11 PM
Replies: 6
Views: 5,318
Posted By provirus
Have a look at your intensities of the first 25...

Have a look at your intensities of the first 25 cycles (e.g. in the sequence analysis viewer) (which might not only be a problem for amplicon sequencing). Below a quote from a Illumina white paper:
...
Forum: Sample Prep / Library Generation 10-18-2017, 01:05 PM
Replies: 3
Views: 1,877
Posted By provirus
It would also be informative with bioanalyzer...

It would also be informative with bioanalyzer profiles showing: a) input (the 200 ng) alone and no library prep, b) library prep with input but no adapters, c) library prep with adapter but no input,...
Forum: Illumina/Solexa 10-18-2017, 12:37 PM
Replies: 12
Views: 2,874
Posted By provirus
Hi Austin. Is it correctly understood that you...

Hi Austin. Is it correctly understood that you had the low diversity regions/patches offset from one another by using 2+ different adapter/amplicon ends (effectively 'moving' the patches relatively...
Forum: Illumina/Solexa 10-18-2017, 10:41 AM
Replies: 12
Views: 2,874
Posted By provirus
Hi Austin, interestingly. I will have a look in...

Hi Austin,
interestingly. I will have a look in the folders, and see if it makes any sense. You are right that PhiX spike would help. We are testing this at 35-45% at the expense of...
Forum: Illumina/Solexa 10-18-2017, 09:12 AM
Replies: 12
Views: 2,874
Posted By provirus
Thanks. We will indeed approach Illumina with...

Thanks. We will indeed approach Illumina with this. I wanted to know if some users had experience with this, and also to learn about the "inner workings" of the programs that control the runs - it is...
Forum: Illumina/Solexa 10-17-2017, 12:44 PM
Replies: 12
Views: 2,874
Posted By provirus
Making a custom run recipe on nextseq 500

Is it possible to design a complete custom run recipe by yourself on a Nextseq500. For instance I am considering a PE sequence run were some specific cycles are chemistry-only (with no pictures...
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