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Forum: Illumina/Solexa 04-15-2015, 09:19 AM
Replies: 3
Views: 2,195
Posted By Ayaka
Inconsistency between 16s sequencing with Illumina and qPCR

Hi all, I would like to ask your opinion about inconsistent results regarding the abundance of certain bacteria between sequence data and qPCR using the same DNA samples.
I have 2 treatments (A...
Forum: Bioinformatics 03-07-2015, 04:21 PM
Replies: 0
Views: 1,149
Posted By Ayaka
Design for normalization using DESeq

Hi all, I am analyzing 16S sequenced data of human fecal samples. I proccessed my data with qiime and have been using the R package phyloseq for the data analysis. I wish to use the phyloseq to DESeq...
Forum: Sample Prep / Library Generation 10-15-2014, 10:58 AM
Replies: 3
Views: 1,977
Posted By Ayaka
Hi Baseless thank you for answering. We indeed...

Hi Baseless thank you for answering. We indeed get a PCR product of the appropriate size. A person from a sequencing center told me that as long as the smeared bands are above the size of the PCR...
Forum: Sample Prep / Library Generation 09-24-2014, 11:50 AM
Replies: 3
Views: 1,977
Posted By Ayaka
Question Genomic DNA integrity importance in amplicon sequencing

Hi there, in my lab we are going to be sequencing 16S with Miseq from stool samples and we are currently in the DNA extraction step.

We are using Qiagen's QiAmp DNA stool mini kit and have...
Forum: Sample Prep / Library Generation 05-08-2014, 08:08 PM
Replies: 4
Views: 2,272
Posted By Ayaka
Thank you all for your answers! I have a very...

Thank you all for your answers! I have a very clear understanding of my queries now. I am truly glad I found this forum!
Forum: Sample Prep / Library Generation 05-07-2014, 04:46 PM
Replies: 4
Views: 2,272
Posted By Ayaka
Question Newbie:Use of kit for library prep.Why can I just amplify using index,adaptor seq.?

Hi there, I am very new in the sequencing world and I am gathering the needed info to start the firs sequencing project of 16S amplicons with MiSeq in our lab.
My question is regarding the library...
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