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Forum: Bioinformatics 12-11-2013, 05:26 AM
Replies: 0
Views: 1,270
Posted By sc10021
Post weird kmer in FastQC plot

I have performed whole exome sequencing using the 62Mb Nextera Exome Enrichment from Illumina. I have really weird results where a lot of the "aligned" reads have too many variants and so have...
Forum: Sample Prep / Library Generation 03-12-2013, 10:08 PM
Replies: 12
Views: 11,481
Posted By sc10021
Thank you.

Thank you.
Forum: Sample Prep / Library Generation 03-08-2013, 10:50 AM
Replies: 12
Views: 11,481
Posted By sc10021
ampure beads

Left my beads on bench overnight. Is it still OK to use?
Forum: Illumina/Solexa 02-16-2011, 04:26 AM
Replies: 7
Views: 3,299
Posted By sc10021
hi pmiguel can you elaborate on the band and...

hi pmiguel

can you elaborate on the band and what it is please? I see it in my RNA and DNA preps as well
TQ
Forum: Illumina/Solexa 09-14-2010, 11:45 PM
Replies: 8
Views: 3,703
Posted By sc10021
problem with mRNA multiplex libraries

I have been trying to generate mRNA-SEQ multiplex libraries using the multiplex kit from Illumina but my end repair, dA-tailing and ligation reagents are from NEB labs. I have no problem generating...
Forum: RNA Sequencing 08-20-2010, 06:15 AM
Replies: 0
Views: 1,312
Posted By sc10021
Supercript III SuperMix

Hi

Anyone tried using the Superscript III Super mix to generate the first strand cDNA for RNA-seq libraries? The supermix contains the RT reaction buffer and RT enzyme mix.
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