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Forum: Bioinformatics 08-06-2014, 03:17 AM
Replies: 7
Views: 4,280
Posted By mihuzx
hi velt, thank you very much. I have...

hi velt,
thank you very much.
I have tried it with my data. the SERE score is 5.8.
and another pair replication is about 3.3
is this too high? or any sugguestions ?
by the...
Forum: Bioinformatics 08-05-2014, 10:47 PM
Replies: 7
Views: 4,280
Posted By mihuzx
thank you for your advice, I removed all genes...

thank you for your advice,
I removed all genes < 10 and calculated the spearman correlation, but it still only about 0.93.
and I calculate spearson crrelation with genes < 1RPKM ,it didn't change....
Forum: Bioinformatics 08-05-2014, 04:52 PM
Replies: 7
Views: 4,280
Posted By mihuzx
Unhappy RNA-seq bio-replication with low correlation

Hi!
I sequeced two biological replicates for one condition with Hiseq 2000 platfom.
but the typical R(Pearson) correlation of gene expression(Raw Count) between two biological replicates is...
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