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Forum: Vendor Forum 11-24-2016, 05:45 PM
Replies: 108
Views: 65,167
Posted By Michal2213
NextSeq suitable for allele-specific analysis?

Thank you for reply! I would do the same but with NextSeq we are getting data within a week as we have direct access and with HiSeq2000 in the sequencing core one need to wait in many cases more than...
Forum: Vendor Forum 11-24-2016, 08:40 AM
Replies: 108
Views: 65,167
Posted By Michal2213
NextSeq suitable for allele-specific analysis?

Do you think that NextSeq would be suitable for allele-specific analysis? I am using mouse cells with hybrid genome and sort the reads belonging to different alleles based on SNP content. So far I...
Forum: Illumina/Solexa 06-02-2015, 03:03 AM
Replies: 0
Views: 1,333
Posted By Michal2213
Illumina Hiseq v3 versus v4 chemistry for whole genome bisulfite sequencing

Hi,

does anybody have experience in using illumina v4 chemistry or Hiseq X chemistry for the whole-genome bisulfite sequencing? Are there any known biases which would make it worse compared to v3?...
Forum: Ion Torrent 04-23-2015, 07:19 AM
Replies: 0
Views: 939
Posted By Michal2213
Ion Proton for allele-specific ChIP-seq, RNA-seq

Hi,

In our lab we are working with allele-specific ChIP-seqs and RNA-seqs. After standard ChIP-seq or RNA-seq protocol we attribute obtained reads to either parental or maternal genome based on...
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