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Forum: General 07-30-2019, 07:35 AM
Replies: 1
Views: 936
Posted By Cistron
Post Example 16S profiling papers with faults in their study design

Hello dear folks,

I know ... this is a weird request. But it's all in the spirit of good science education :D

I am trying to find 16S microbiome papers, with (noticeable) faults in their study...
Forum: Sample Prep / Library Generation 07-15-2019, 11:18 PM
Replies: 6
Views: 737
Posted By Cistron
Ah, super! :) Thanks a bunch! ...

Ah, super! :)



Thanks a bunch!

Anyone experimented with the DIY versions off Thingiverse?
Forum: Sample Prep / Library Generation 07-15-2019, 11:21 AM
Replies: 6
Views: 737
Posted By Cistron
Thanks for your help! Not sure I can get a...

Thanks for your help!

Not sure I can get a purchase order number for that (:D), but do you have examples?

No! That sounds neat. Are there any published protocols around?
Forum: Sample Prep / Library Generation 07-15-2019, 06:18 AM
Replies: 6
Views: 737
Posted By Cistron
Question Removal of <110bp primer-dimers without beads?

Hi all,

I was wondering whether anyone has come across a column-based cleanup kit, which would remove large primers (EMP 16S design) and primer-dimers of around 100bp. Or alternatively, protocols...
Forum: Bioinformatics 12-08-2018, 08:54 AM
Replies: 6
Views: 1,149
Posted By Cistron
huh! I had no idea there was an actual debian...

huh! I had no idea there was an actual debian package for QIIME available. I tried the conda install route, but USEARCH keeps throwing a core dump error when I test the installation.

Ok. I can...
Forum: Bioinformatics 12-07-2018, 12:28 PM
Replies: 6
Views: 1,149
Posted By Cistron
How did you get QIIME working? Is it version 1 or...

How did you get QIIME working? Is it version 1 or 2?
Forum: Illumina/Solexa 02-22-2017, 09:04 AM
Replies: 45
Views: 24,523
Posted By Cistron
Ah, yes - do you mean like this? ...

Ah, yes - do you mean like this?


3'5' 3'5'
| | | |
5'__/ \_____/ \___3'


I guess this could still form a complex bubble.
Forum: Illumina/Solexa 02-21-2017, 12:39 PM
Replies: 45
Views: 24,523
Posted By Cistron
Hi Philip, Thanks for your answer! ...

Hi Philip,

Thanks for your answer!

Right, so "daisy-chains" aren't really extended much, but entangled products. That makes more sense.

The bubble products made sense when I first heard...
Forum: Illumina/Solexa 02-21-2017, 11:00 AM
Replies: 45
Views: 24,523
Posted By Cistron
Hello folks, Interesting thread! I got a lot...

Hello folks,

Interesting thread! I got a lot of high molecular weight smear in my last run as well (following EMP protocols for soil samples).

I'm trying to figure out the details of the...
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