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Forum: Oxford Nanopore 10-05-2014, 06:44 PM
Replies: 47
Views: 18,416
Posted By seqsense
There's no biophysics, it's just plain chemistry....

There's no biophysics, it's just plain chemistry. It might fall out for resequencing but for de novo it's an issue because it's not predictable. There will be an increase in G:T pairing although...
Forum: Oxford Nanopore 10-05-2014, 01:48 AM
Replies: 47
Views: 18,416
Posted By seqsense
Different electrostatics - the structure of...

Different electrostatics - the structure of labeled nucleotides has changed and with that comes different electronic fields which can influence the physical behaviour of the bases especially with...
Forum: Oxford Nanopore 10-04-2014, 01:32 AM
Replies: 47
Views: 18,416
Posted By seqsense
I don't think improved bioinformatics will...

I don't think improved bioinformatics will address the issues with the nextseq chemistry. It's fundamentally flawed and it's apparent the Illumina don't understand the chemistry probably because they...
Forum: General 02-27-2014, 07:49 PM
Replies: 10
Views: 2,381
Posted By seqsense
So far, apart from C, MeC and HOMeC, there's also...

So far, apart from C, MeC and HOMeC, there's also evidence of 5-carboxy-C and 5-formyl-C and their roles in epigenetics.
Forum: General 02-25-2014, 03:28 PM
Replies: 10
Views: 2,381
Posted By seqsense
Hey, don't forget that unless the sequencing is...

Hey, don't forget that unless the sequencing is done on a single cell, which does happen for researching tumour genesis, there will be many, many copies of the DNA already without the need for PCR...
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