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Forum: Sample Prep / Library Generation 04-25-2016, 07:23 AM
Replies: 4
Views: 2,202
Posted By Maflores11
Dear Luc, Thanks for the insight. I'm...

Dear Luc,

Thanks for the insight. I'm curious about the number of PCR cycles you guys are performing with your ChIP samples.
I used 75 nM of adapters for each ChIP sample when doing the library...
Forum: Sample Prep / Library Generation 04-22-2016, 11:32 AM
Replies: 4
Views: 2,202
Posted By Maflores11
Poly AT-stretches in ChIP-seq reads from NextSeq

Hi All,

I submitted 12 samples for ChIP-seq using the NexSeq Platform. Briefly, these samples represent 3 ChIP Biological Replicates:
Mock
Transcription Factor (TF)
Histone H3 (CT)
Input
...
Forum: Literature Watch 02-29-2016, 08:08 PM
Replies: 19
Views: 16,241
Posted By Maflores11
Hi Kathi_seq, This is Miguel Flores (user:...

Hi Kathi_seq,

This is Miguel Flores (user: ashes11), I was unable to login in with my previous account.

In order to minimize COCl2 precipitation (likely due to DTT in NEB4 Buffer), I tried...
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