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Forum: RNA Sequencing 12-12-2016, 03:46 PM
Replies: 2
Views: 2,125
Posted By dwong
NuGEN has a kit that has all the abovementioned...

NuGEN has a kit that has all the abovementioned features, the Ovation SoLo RNA-Seq Kit.
Forum: Illumina/Solexa 11-04-2016, 09:28 AM
Replies: 4
Views: 3,651
Posted By dwong
For specific organism depletion, have you...

For specific organism depletion, have you considered using NuGEN’s model organism kits utilizing our InDA-C technology? For non-model organisms, we offer customization. The way this works is that you...
Forum: Illumina/Solexa 10-25-2016, 10:07 AM
Replies: 14
Views: 4,720
Posted By dwong
I used their Drosophila kit and it knocked down...

I used their Drosophila kit and it knocked down the rRNA quite a bit. Its great that its specific to the organism of choice, but that's just my personal opinion ;)

Darren
Forum: Illumina/Solexa 10-24-2016, 03:55 PM
Replies: 14
Views: 4,720
Posted By dwong
Have you guys ever considered using NuGEN's...

Have you guys ever considered using NuGEN's InDA-C customized depletion? They also have standard depletion kits for human, Drosophila and mouse. The depletion is only performed after the library is...
Forum: Sample Prep / Library Generation 10-07-2016, 03:11 PM
Replies: 6
Views: 3,585
Posted By dwong
Not sure if you have a solution yet but I have...

Not sure if you have a solution yet but I have seen this double hump phenomenon before. Usually what causes this is gDNA contamination or overamplification of your libraries. Try using a solution...
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