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Search: Posts Made By: bhennuy
Forum: Bioinformatics 05-02-2014, 02:24 AM
Replies: 3
Views: 1,827
Posted By bhennuy
Hello, Did you try to compare the three...

Hello,
Did you try to compare the three conditions in the same process with cuffdiff ?
As explained, Cuffdiff should support that with "cuffdiff sample1 sample2 sample3". The 3 conditions should be...
Forum: RNA Sequencing 03-03-2014, 03:56 AM
Replies: 0
Views: 1,018
Posted By bhennuy
Cuffdiff with 3 experimental conditions

Hello,
I used Cuffdiff 2.1.1 to analyse and compare 3 different experimental conditions, all together. I followed the command mentionned in the manual : cuffdiff <options>...
Forum: General 12-05-2012, 07:43 AM
Replies: 5
Views: 13,894
Posted By bhennuy
Thank you TonyBrooks for the info, I will read it...

Thank you TonyBrooks for the info, I will read it carefully. Do you know why Illumina do not provide more i5 indexes to increase the number of multiplexes ?
Forum: General 12-05-2012, 05:13 AM
Replies: 5
Views: 13,894
Posted By bhennuy
indexes sequence for multiplexed > 96

Hello,
We are interested in using dual indexing strategy for multiplexing more than 384 samples in one run on MiSeq instrument. Could we use the 48 indexes (6 bases) provided by Illumina for Truseq...
Forum: Sample Prep / Library Generation 01-26-2011, 11:55 PM
Replies: 23
Views: 7,747
Posted By bhennuy
DSN normalization with TruSeq kits

Hi,
Has anyone already try the DSN normalization with the new RNA TruSeq kits ?
How to fragment the total RNA and to link this step with the Truseq protocol?
Forum: RNA Sequencing 01-26-2011, 12:55 AM
Replies: 0
Views: 1,422
Posted By bhennuy
DSN noramlization with TruSeq kits

Hi,
Does anyone have already tried the DSN normalisation with the new Illumina RNA TruSeq kit ?
How to fragment RNA before to start the cDNA synthesis ?

Thanks for your comment
Benoit
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