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Forum: RNA Sequencing 11-13-2018, 06:41 AM
Replies: 1
Views: 1,360
Posted By kc3228
It may help to have more information. Could you...

It may help to have more information. Could you post bioanalyzer traces for the RNA? How many cycles of PCR did you use? What did your final library bioanalyzers look like?
Forum: RNA Sequencing 08-22-2018, 05:56 AM
Replies: 1
Views: 1,649
Posted By kc3228
It looks like you have an error on the lane and...

It looks like you have an error on the lane and the ribosomal peaks are not being called correctly. You'll need to manually assign the 18s and 28s peaks, and address any errors in the lane before it...
Forum: RNA Sequencing 07-19-2018, 11:42 AM
Replies: 1
Views: 1,129
Posted By kc3228
Your best bet is probably to add trizol or...

Your best bet is probably to add trizol or similar directly to the plates and scrape the cells off, then extract RNA. Other than that, try to keep things cold.
Forum: RNA Sequencing 04-05-2018, 06:07 AM
Replies: 1
Views: 1,491
Posted By kc3228
Did your colleague measure proteins A and B only...

Did your colleague measure proteins A and B only via Western or also at the mRNA level? It could be that the effects of the shRNA are different on RNA than they are on the protein. Further, if the...
Forum: Illumina/Solexa 03-07-2018, 11:20 AM
Replies: 7
Views: 1,890
Posted By kc3228
The two lasers are red and green. Red is C, green...

The two lasers are red and green. Red is C, green is T. A is labeled with both red and green, so shows up in both. G is dark (unlabeled), so a lack of signal is interpreted as G.
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