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Forum: Sample Prep / Library Generation 05-21-2018, 07:59 AM
Replies: 13
Views: 4,122
Posted By DPVT
So for our qPCR we do 20,000x dilutions of our...

So for our qPCR we do 20,000x dilutions of our pools to achieve a dilution in the middle of the stnd curve (around 0.2pM) and do two dilutions of 1000x and 5000x of the internal standard 0. however...
Forum: Sample Prep / Library Generation 05-04-2018, 09:36 AM
Replies: 13
Views: 4,122
Posted By DPVT
Hey Chuanwu and MU Core, We've started to...

Hey Chuanwu and MU Core,

We've started to include KAPA standard 0 (from their illumina quantitation kit) to our qPCR assays and have adjusted the qPCR concentrations of our pools accordingly. In...
Forum: Sample Prep / Library Generation 12-01-2017, 05:06 AM
Replies: 13
Views: 4,122
Posted By DPVT
qPCR/ Qubit discrepency

Our lab has often encountered trouble quantifying our Illumina pools. We sequence all manner of samples and find that some pools cluster more robustly then other others even when loaded at the same...
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