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Forum: Sample Prep / Library Generation 12-21-2012, 01:41 PM
Replies: 18
Views: 13,698
Posted By Papaveraceae
Johan, I am interested in your IP protected...

Johan,

I am interested in your IP protected magnetic bead with active DNase on the surface. Is the information available online if not please let me know more about it via email.

Thanks,
...
Forum: RNA Sequencing 12-21-2012, 12:58 PM
Replies: 31
Views: 28,962
Posted By Papaveraceae
Phillip, I made no mention of my RNA...

Phillip,

I made no mention of my RNA degrading after DNAse treatment, in fact I havent treated them with DNAse at all yet, on the contrary the RIN of 9.3-9.2 from my non-DNAsed RNA (extracted...
Forum: RNA Sequencing 12-21-2012, 12:53 PM
Replies: 31
Views: 28,962
Posted By Papaveraceae
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Forum: Sample Prep / Library Generation 12-21-2012, 12:47 PM
Replies: 18
Views: 13,698
Posted By Papaveraceae
Has anyone used RNAzol, from Molecular Research...

Has anyone used RNAzol, from Molecular Research Center, they make the claim that DNAse is not required after extraction. See the link: http://www.mrcgene.com/rnazol.htm

Dave
Forum: RNA Sequencing 12-21-2012, 11:29 AM
Replies: 31
Views: 28,962
Posted By Papaveraceae
Zymo Columns

Phillip,

I'm quite new to RNA work but I have managed to get some pretty good results on my pre-DNased samples, see image. I recently read your comment about using the Zymo spin columns to clean...
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