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Search: Posts Made By: Lmathew
Forum: General 08-11-2017, 09:01 AM
Replies: 6
Views: 4,771
Posted By Lmathew
Agilent 4200 TapeStation Instrument / PerkinElmer LabChip GX Touch 24 / AATI Fragm

Looking for inputs on the "nucleic acid analyzers" for NGS studies. Our lab is planning to purchase either a Agilent 4200 TapeStation Instrument or PerkinElmer LabChip GX Touch 24 or AATI Fragment...
Forum: Pacific Biosciences 04-20-2017, 10:25 AM
Replies: 0
Views: 1,320
Posted By Lmathew
retain barcodes in the output file after isoseq analysis in SMRTlink

I have a sample set that were barcoded uniquely for each sample. After we did IsoSeq analysis with barcoding options in SMRTlink, I obtained reads that were sorted by barcodes. However, on further...
Forum: Pacific Biosciences 03-23-2017, 10:30 AM
Replies: 4
Views: 1,878
Posted By Lmathew
Thank you, rhall. I do have decent full length...

Thank you, rhall. I do have decent full length reads. I appreciate your help.
Forum: Pacific Biosciences 03-23-2017, 09:06 AM
Replies: 4
Views: 1,878
Posted By Lmathew
Thank you for your response! PCR product...

Thank you for your response!

PCR product from each sample was ~ 5 kb and unique PacBio barcode was added directly to gene specific primer and each sample cDNA was PCR amplified using specific...
Forum: Pacific Biosciences 03-22-2017, 07:31 AM
Replies: 4
Views: 1,878
Posted By Lmathew
PacBio data analysis help requested...

I am a newbie into PacBio sequencing.:) I currently have data from a targeted sequencing study of a specific transcript.

What we have is data from 3 groups of individuals (10-homozygous resistant,...
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