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Forum: Sample Prep / Library Generation 06-25-2019, 05:47 AM
Replies: 12
Views: 866
Posted By beancat
So I cut my reaction volume considerably with a...

So I cut my reaction volume considerably with a lot of other deviations from the normal protocol (including PCR-free) so I don't think a direct comparison is useful. The only thing I can say is that...
Forum: Sample Prep / Library Generation 05-28-2019, 09:42 AM
Replies: 12
Views: 866
Posted By beancat
I've used Kapa Hyperplus 3-4 times a week for...

I've used Kapa Hyperplus 3-4 times a week for well over a year and what causes me to decrease yield or lose samples have almost always been the the frag enzyme activity or sometimes beads.
...
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