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Forum: 454 Pyrosequencing 02-24-2011, 04:24 AM
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Posted By knakahig
Question DNA amount for making Lib-L from PCR fragment

Hi all,
I am going to make ligation library from PCR fragments.
Roche recommend to use 0.5 microgram of DNA (10^11 molecules as 500 bp) for this and after purification it becomes around 10^9 ends...
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