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Forum: Sanger/Dye Terminator 12-29-2015, 07:54 PM
Replies: 3
Views: 5,811
Posted By mohd2b
Hi MU Core iam using BigDye v1.1 chemistry.

Hi MU Core

iam using BigDye v1.1 chemistry.
Forum: Sanger/Dye Terminator 12-28-2015, 09:23 PM
Replies: 3
Views: 5,811
Posted By mohd2b
Rerun of cycle sequencing

Hi All

Recently I have run a cycle sequencing reaction, however the run was stopped due to a fatal error in the thermocycler. it stopped at cycle 8 of 25 cycles for approximately an hour till I...
Forum: Sanger/Dye Terminator 12-16-2015, 09:37 PM
Replies: 1
Views: 5,694
Posted By mohd2b
here are some image of my sequence

here are some image of my sequence
Forum: Sanger/Dye Terminator 12-14-2015, 09:13 PM
Replies: 1
Views: 5,694
Posted By mohd2b
3130 genetic analyzer raw data range

hi

Well for those who have used 3130 genetic analyzer, when you look in the raw data of a run , how do you interrupt the results?

in my case , most of the time I got a raw data signal...
Forum: Sanger/Dye Terminator 12-14-2015, 08:53 PM
Replies: 4
Views: 6,540
Posted By mohd2b
Viroseq sequencing

hi Ivoalexiev

Well so far we find out that we have problem in measuring the right concentration of the PCR purified products, which is very critical in cycle sequencing reactions . Unfortunately,...
Forum: Sanger/Dye Terminator 11-11-2015, 01:58 AM
Replies: 4
Views: 6,540
Posted By mohd2b
ViroSeq sequencing

hi Ivoalexiev

Good to know that you are using ViroSeq for resistance studies. Iam also using it. however we facing problem with getting good and clean sequence.

Is it possible to share your...
Forum: Sanger/Dye Terminator 11-19-2014, 07:11 PM
Replies: 5
Views: 3,136
Posted By mohd2b
thanks guys well my template is pol HIV gene...

thanks guys

well my template is pol HIV gene

this is kit based methods. I use Viroseq kit to test for resistance. I have used it in my training in Germany and now iam optimizing it here in our...
Forum: Sanger/Dye Terminator 11-18-2014, 08:59 PM
Replies: 5
Views: 3,136
Posted By mohd2b
thanks for reply sequencingfan Well after...

thanks for reply sequencingfan

Well after receiving the analysis software we have seen that there are some setting we need to modify in analysis protocol we used.
We managed to get a quite good...
Forum: Sanger/Dye Terminator 11-09-2014, 09:21 PM
Replies: 5
Views: 3,136
Posted By mohd2b
low intensity signal with peaks under peaks

Hello
well iam doing sanger sequence for samples that used for resistance analysis. the bigdye chemistry is v1.1

after getting the sequence trace files raw data has shown low signal intensity...
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