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Forum: Illumina/Solexa 03-13-2015, 10:23 AM
Replies: 58
Views: 52,728
Posted By Wei-HD
Thanks fanli! I have checked my reads. Seq_1.txt...

Thanks fanli! I have checked my reads. Seq_1.txt is from the antisense strand Seq_2.txt is from the sense strand on UCSC.

Then I do tophat --library-type fr-firststrand seq_1.txt seq_2.txt and ...
Forum: Illumina/Solexa 03-13-2015, 10:08 AM
Replies: 58
Views: 52,728
Posted By Wei-HD
Hi all, I have 2 x 50 bp paired end data,...

Hi all,

I have 2 x 50 bp paired end data, library preparation from NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®, dUTP method.

Data as: seq_1.txt and seq_2.txt

From the...
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