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Forum: Illumina/Solexa 06-05-2013, 12:33 PM
Replies: 58
Views: 52,728
Posted By carmeyeii
Red face :o I know - my bad. i just wanted to say that...

:o
I know - my bad. i just wanted to say that it is the original RNA molecule, without it being "actually the RNA molecule", which of course we all know because this is cDNA we're talking about.;)
Forum: Illumina/Solexa 06-05-2013, 12:09 PM
Replies: 58
Views: 52,728
Posted By carmeyeii
No prob. OK, so just to make sure I'm on the...

No prob.
OK, so just to make sure I'm on the right track -- the sequence that is "spit out" by the sequencer is the actual sequence as seen by the camera... i.e, no base translation, just raw...
Forum: Illumina/Solexa 06-05-2013, 11:54 AM
Replies: 58
Views: 52,728
Posted By carmeyeii
C

kcarr, I thought ScriptSeq's directional protocol is actually tag-based?
I think it doesn't involver dUTP, does it? -- at least ScriptSeq v2 ...
Forum: Illumina/Solexa 06-04-2013, 08:51 AM
Replies: 58
Views: 52,728
Posted By carmeyeii
Is this true? If that is the case, then ...

Is this true?

If that is the case, then

a) the illustration above should portray the complementary strand to the RNA being used as template to synthesize (and read) the actual mRNA molecule by...
Forum: Illumina/Solexa 09-06-2012, 07:57 PM
Replies: 58
Views: 52,728
Posted By carmeyeii
Thank you amitm. After re-reading it is now...

Thank you amitm.

After re-reading it is now clear that they did not use any strand-specific protocol.

Thanks for your help!

Carmen :)
Forum: Illumina/Solexa 09-05-2012, 08:23 PM
Replies: 58
Views: 52,728
Posted By carmeyeii
Hello! I am analyzing a dataset which, from...

Hello!

I am analyzing a dataset which, from the Methods section, appears to be directional:



It was generated on the GaxII.

After reading this post, I think it is safe to say that the...
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