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Forum: Sample Prep / Library Generation 04-16-2018, 03:08 AM
Replies: 0
Views: 1,480
Posted By EGraf
DNA vs. RNA baits for enrichment

Dear all,

does anyone of you knows why Agilent is working with RNA baits and Illumina with DNA baits for exome enrichment? Is this caused due to patent rights or are there advantages and...
Forum: Sample Prep / Library Generation 09-30-2015, 02:45 AM
Replies: 3
Views: 1,740
Posted By EGraf
Quantifying whole genome PCR-free libs without qPCR

Hi everybody,

I would like to run whole genome sequencing in a high throughput manner.

Libraries are prepared using Illuminas TruSeq PCR-free protocol and chemistry.

The time and cost...
Forum: Sample Prep / Library Generation 04-08-2015, 03:06 AM
Replies: 1
Views: 2,641
Posted By EGraf
ATACseq_which size for calculation of concentration using qPCR

Hi everybody,

I have to sequence an ATAc seq pool on our HiSeq. It's the first time I have such a library with broad size range ranging from 150 bp up to 7 kb. I would like to quantify the pool...
Forum: Sample Prep / Library Generation 03-31-2015, 12:06 AM
Replies: 0
Views: 1,942
Posted By EGraf
ChIP seq_why size selection pre PCR?

Hi everybody,

this might be a stupid question but I did not get it. Why is the size selection step in the ChIP library prep process prior PCR enrichment?

I suppose using the whole amount of...
Forum: Sample Prep / Library Generation 07-09-2014, 05:02 AM
Replies: 3
Views: 2,136
Posted By EGraf
Thanks for reply. I used 2 g DNA of human and...

Thanks for reply. I used 2 g DNA of human and bacterial DNA, respectively. I excepted nearly the same concentration after shearing. Do I have an error in reasoning :confused:

I would like to use...
Forum: Sample Prep / Library Generation 07-09-2014, 03:35 AM
Replies: 3
Views: 2,136
Posted By EGraf
Covaris fragmentation of small genomes

Hi everyone,

today I tried shearing of a bacterial genome (1,7 Mb). I checked DNA integrity previously and it looks fine with no degradation. I used our standard settings for 550 bp fragment size....
Forum: Sample Prep / Library Generation 11-21-2013, 06:05 AM
Replies: 1
Views: 1,408
Posted By EGraf
Search for Caliper GX expiriences

Hi everybody,

for quality and quantity control of our exome libraries we are using the Caliper GX system.

Since the beginning of usage we had some issues with the machine.
There are...
Forum: Sample Prep / Library Generation 10-26-2011, 06:06 AM
Replies: 0
Views: 2,456
Posted By EGraf
Sure Select pre-Hyb lib storage

Hi everybody,

we are working with the Sure Select target enrichment kit. I stopped the sample prep and had store the adapter ligated samples (-20C) prior the hybridization. I request Agilent how...
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