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Forum: Sample Prep / Library Generation 10-18-2019, 01:32 PM
Replies: 2
Views: 870
Posted By JackieM
I work with trypanosomes, so I used the Muller...

I work with trypanosomes, so I used the Muller 2018 protocol for AtacSeq, but I used the Q5 master mix (we prep a lot of standard NGS libraries, so it's what I had lying around) and had great results.
Forum: Sample Prep / Library Generation 10-18-2019, 01:05 PM
Replies: 2
Views: 693
Posted By JackieM
If it's daisy chaining/bubble product, you should...

If it's daisy chaining/bubble product, you should be able to take a small amount, re-amplify it for a few cycles and the secondary peak will go away. As far as over-amplification goes- I usually use...
Forum: Sample Prep / Library Generation 05-29-2017, 03:23 PM
Replies: 10
Views: 5,955
Posted By JackieM
I don't mean to spam the thread, but this is a...

I don't mean to spam the thread, but this is a great point and reminded me of something ONT tech support advised me: If you're not sure, it's better to add your RSB before the ethanol has dried as...
Forum: Sample Prep / Library Generation 05-27-2017, 03:23 PM
Replies: 10
Views: 5,955
Posted By JackieM
All this talk of gDNA quantification gave me a...

All this talk of gDNA quantification gave me a thought- when you add your buffer to the beads and incubate it, is your concentration/molecular weight high enough that you can see the DNA in solution?...
Forum: Sample Prep / Library Generation 05-25-2017, 07:46 PM
Replies: 10
Views: 5,955
Posted By JackieM
I second this. I've found that the more you mix...

I second this. I've found that the more you mix the beads with your DNA, you can actually see the beads get lodged into your sample. I would make sure not to mix the beads for too long, not to...
Forum: Sample Prep / Library Generation 08-06-2015, 08:19 PM
Replies: 3
Views: 3,294
Posted By JackieM
Update

We decided to go with KAPA as it's what I'm familiar with, and it has been working beautifully. The kit even survived our freezer going down over a weekend! A previous colleague of mine- you guys may...
Forum: Illumina/Solexa 05-11-2015, 01:50 PM
Replies: 1
Views: 1,918
Posted By JackieM
Did you ever find a solution to this? I'm looking...

Did you ever find a solution to this? I'm looking to fragment some larger quantities as well. It'd be really nice -cost wise- if we were able to do it in a single tube.

Best,
Jackie
Forum: Sample Prep / Library Generation 04-09-2015, 02:59 PM
Replies: 3
Views: 3,294
Posted By JackieM
Thanks for the info! That's very helpful!

Thanks for the info! That's very helpful!
Forum: Sample Prep / Library Generation 04-08-2015, 06:59 PM
Replies: 3
Views: 3,294
Posted By JackieM
Kapa vs NEBnext for ChIPSeq?

Hi Seqanswers!
Forgive me if theres already a post on this (I saw a post comparing Truseq ChIP kit with NEBnext, but nothing on Kapa) but does anyone have any experience using a Kapa hyper or Kapa...
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