Forum: Sample Prep / Library Generation
10-18-2019, 01:32 PM
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Replies: 2
Views: 870
I work with trypanosomes, so I used the Muller...
I work with trypanosomes, so I used the Muller 2018 protocol for AtacSeq, but I used the Q5 master mix (we prep a lot of standard NGS libraries, so it's what I had lying around) and had great results.
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Forum: Sample Prep / Library Generation
10-18-2019, 01:05 PM
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Replies: 2
Views: 693
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Forum: Sample Prep / Library Generation
05-29-2017, 03:23 PM
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Replies: 10
Views: 5,955
I don't mean to spam the thread, but this is a...
I don't mean to spam the thread, but this is a great point and reminded me of something ONT tech support advised me: If you're not sure, it's better to add your RSB before the ethanol has dried as...
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Forum: Sample Prep / Library Generation
05-27-2017, 03:23 PM
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Replies: 10
Views: 5,955
All this talk of gDNA quantification gave me a...
All this talk of gDNA quantification gave me a thought- when you add your buffer to the beads and incubate it, is your concentration/molecular weight high enough that you can see the DNA in solution?...
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Forum: Sample Prep / Library Generation
05-25-2017, 07:46 PM
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Replies: 10
Views: 5,955
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Forum: Sample Prep / Library Generation
08-06-2015, 08:19 PM
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Replies: 3
Views: 3,294
Update
We decided to go with KAPA as it's what I'm familiar with, and it has been working beautifully. The kit even survived our freezer going down over a weekend! A previous colleague of mine- you guys may...
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Forum: Illumina/Solexa
05-11-2015, 01:50 PM
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Replies: 1
Views: 1,918
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Forum: Sample Prep / Library Generation
04-09-2015, 02:59 PM
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Replies: 3
Views: 3,294
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Forum: Sample Prep / Library Generation
04-08-2015, 06:59 PM
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Replies: 3
Views: 3,294
Kapa vs NEBnext for ChIPSeq?
Hi Seqanswers!
Forgive me if there’s already a post on this (I saw a post comparing Truseq ChIP kit with NEBnext, but nothing on Kapa) but does anyone have any experience using a Kapa hyper or Kapa...
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