SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 2 of 2
Search took 0.00 seconds.
Search: Posts Made By: MzwaneleN
Forum: Sample Prep / Library Generation 10-09-2020, 03:07 PM
Replies: 0
Views: 813
Posted By MzwaneleN
PCR validation of ATAC-seq library

I have performed PCR validation of an ATAC-seq library targeting known/expected accessible chromatin regions (100bp) but 90% of the products have a smearing banding pattern. Do you perhaps know what...
Forum: Sample Prep / Library Generation 09-19-2020, 09:34 PM
Replies: 0
Views: 1,202
Posted By MzwaneleN
Contamination of E.coli reads in ATAC-seq library

Hi All,

I have recently run an ATAC-seq library on PBMCs that came back with 82% reads mapping to E.coli and only 5% mapping to the human genome.

The sequencing run was overclustered but had...
Showing results 1 to 2 of 2

 


All times are GMT -8. The time now is 01:51 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO