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Forum: Sample Prep / Library Generation 10-09-2020, 03:07 PM
Replies: 0
Views: 813
Posted By MzwaneleN
PCR validation of ATAC-seq library

I have performed PCR validation of an ATAC-seq library targeting known/expected accessible chromatin regions (100bp) but 90% of the products have a smearing banding pattern. Do you perhaps know what...
Forum: Sample Prep / Library Generation 09-19-2020, 09:34 PM
Replies: 0
Views: 1,202
Posted By MzwaneleN
Contamination of E.coli reads in ATAC-seq library

Hi All,

I have recently run an ATAC-seq library on PBMCs that came back with 82% reads mapping to E.coli and only 5% mapping to the human genome.

The sequencing run was overclustered but had...
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